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Purification of recombinant proteins by fusion with thermally-responsive polypeptides
Elastin-like polypeptides (ELPs) undergo a reversible, inverse phase transition. Below their transition temperature (Tt), ELPs are soluble in water, but when the temperature is raised above Tt, phase… Expand
Tumor vascular permeability, accumulation, and penetration of macromolecular drug carriers.
- M. Dreher, W. Liu, C. R. Michelich, M. Dewhirst, F. Yuan, A. Chilkoti
- Medicine, Biology
- Journal of the National Cancer Institute
- 1 March 2006
BACKGROUND Delivery of anticancer therapeutic agents to solid tumors is problematic. Macromolecular drug carriers are an attractive alternative drug delivery method because they appear to target… Expand
Genetically encoded synthesis of protein-based polymers with precisely specified molecular weight and sequence by recursive directional ligation: examples from the elastin-like polypeptide system.
We report a new strategy for the synthesis of genes encoding repetitive, protein-based polymers of specified sequence, chain length, and architecture. In this stepwise approach, which we term… Expand
Self-assembling chimeric polypeptide-doxorubicin conjugate nanoparticles that abolish tumors after a single injection
- J. MacKay, M. Chen, Jonathan R. McDaniel, W. Liu, Andrew J. Simnick, A. Chilkoti
- Materials Science, Medicine
- Nature materials
- 8 November 2009
New strategies to self-assemble biocompatible materials into nanoscale, drug-loaded packages with improved therapeutic efficacy are needed for nanomedicine. To address this need, we developed… Expand
Quantification of the effects of chain length and concentration on the thermal behavior of elastin-like polypeptides.
At a specific temperature, elastin-like polypeptides (ELPs) undergo a sharp solubility transition that can be exploited in a variety of applications in biotechnology and medicine. The temperature of… Expand
Overcoming limitations in nanoparticle drug delivery: triggered, intravascular release to improve drug penetration into tumors.
Traditionally, the goal of nanoparticle-based chemotherapy has been to decrease normal tissue toxicity by improving drug specificity to tumors. The enhanced permeability and retention effect can… Expand
Expression and purification of recombinant proteins from Escherichia coli: Comparison of an elastin‐like polypeptide fusion with an oligohistidine fusion
- K. Trabbic-Carlson, L. Liu, B. Kim, A. Chilkoti
- Chemistry, Medicine
- Protein science : a publication of the Protein…
- 1 December 2004
Thermally responsive elastin like polypeptides (ELPs) can be used to purify proteins from Escherichia coli culture when proteins are expressed as a fusion with an ELP. Nonchromatographic purification… Expand
Protein Purification by Fusion with an Environmentally Responsive Elastin‐Like Polypeptide: Effect of Polypeptide Length on the Purification of Thioredoxin
Elastin‐like polypeptides (ELPs) undergo a reversible, soluble‐to‐insoluble phase transition in aqueous solution upon heating through a characteristic transition temperature (Tt). Incorporating a… Expand
Controlled-reflectance surfaces with film-coupled colloidal nanoantennas
Efficient and tunable absorption is essential for a variety of applications, such as designing controlled-emissivity surfaces for thermophotovoltaic devices, tailoring an infrared spectrum for… Expand
Plasmonic detection of a model analyte in serum by a gold nanorod sensor.
We describe the fabrication of a label-free, chip-based biosensor based on the localized surface plasmon resonance (LSPR) of gold nanorods. Gold nanorods were chemisorbed onto a… Expand