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Analysis of bacterial community structure in bulk soil by in situ hybridization
In situ hybridization with rRNA-targeted, fluorescent (Cy3-labeled) oligonucleotide probes was used to analyze bacterial community structure in ethanol- or paraformaldehyde-fixed bulk soil afterExpand
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Differential enumeration and in situ localization of microorganisms in the hindgut of the lower termite Mastotermes darwiniensis by hybridization with rRNA-targeted probes
Abstract We examined the abundance and spatial distribution of major phylogenetic groups of the domain Bacteria in hindguts of the Australian lower termite Mastotermes darwiniensis by using in situExpand
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Molecular phylogeny of litostome ciliates (Ciliophora, Litostomatea) with emphasis on free-living haptorian genera.
The monophyly of the litostomes was tested using nine newly sequenced and four previously unpublished small subunit ribosomal RNA (SSrRNA) gene sequences from free-living Haptoria as well as fromExpand
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Tracing toluene-assimilating sulfate-reducing bacteria using 13C-incorporation in fatty acids and whole-cell hybridization
Polar lipid-derived fatty acids (PLFA) commonly found in sulfate-reducing bacteria were detected in high abundance in the sediment harvested from a monitoring well of a petroleum-hydrocarbonExpand
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Analysis of broad-scale differences in microbial community composition of two pristine forest soils.
Broad-scale differences in soil microbial community composition were analyzed in two contrasting soils using DNA reassociation and % G + C profiles for analysis on the community-level, and filter-Expand
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Genetic diversity in chrysophytes: Comparison of different gene markers
Chrysophytes are a diverse group of protists belonging to the Stramenopiles. They encompass a broad range of nutrition strategies (phototrophy, mixotrophy and heterotrophy) and morphological formsExpand
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Cyst formation is a characteristic feature of chrysophytes. Cyst morphology is used for species identification, and the distribution of cysts in sediment cores is used to infer past climateExpand
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Use of isotopic and molecular techniques to link toluene degradation in denitrifying aquifer microcosms to specific microbial populations
Abstract. Microcosms were inoculated with sediments from both a petroleum-hydrocarbon (PHC)-contaminated aquifer and from a nearby pristine aquifer and incubated under anoxic denitrifying conditionsExpand
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SSU rRNA gene variation resolves population heterogeneity and ecophysiological differentiation within a morphospecies (Stramenopiles, Chrysophyceae)
The objective of this study was to analyze the extent of ecophysiological and molecular homogeneity, or heterogeneity, of distinct protist populations and to test for correlations between molecularExpand
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Application of Qualitative and Quantitative Real-Time PCR, Direct Sequencing, and Terminal Restriction Fragment Length Polymorphism Analysis for Detection and Identification of Polymicrobial 16S rRNA
ABSTRACT Qualitative and quantitative 16S rRNA gene-based real-time PCR and direct sequencing were applied for rapid detection and identification of bacterial DNA (bactDNA) in 356 ascites samples.Expand
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