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Environmental Biotechnology: Principles and Applications
This paper presents a meta-anatomy of the bioremediation and biodegradation processes in the context of wastewater treatment, which has implications for human health and the environment.
Organization and nucleotide sequence determination of a gene cluster involved in 3-chlorocatechol degradation.
Three critical enzymes catechol oxygenase II (chlorocatechol dioxygenase), muconate cycloisomerase II, and dienelactone hydrolase, are involved in the degradation of chlorocatechols, which are
Role of alginate lyase in cell detachment of Pseudomonas aeruginosa
It appears that high-molecular-weight alginate polymers are required to efficiently retain the bacteria within the growth film, and a possible role for the lyase in the development of bacterial growth films is suggested.
Sigma factor-anti-sigma factor interaction in alginate synthesis: inhibition of AlgT by MucA
Conversion from the nonmucoid to the mucoid phenotype is a typical feature of Pseudomonas aeruginosa strains causing chronic pulmonary infections in cystic fibrosis patients, and inhibiting AlgT-dependent transcription could be the mechanism by which mucA suppresses mucoidy in vivo.
Cloning of genes controlling alginate biosynthesis from a mucoid cystic fibrosis isolate of Pseudomonas aeruginosa
Mucoid strains of Pseudomonas aeruginosa isolated from the sputum of cystic fibrosis patients produce copious quantities of an exopolysaccharide known as alginic acid, which has been difficult to isolate individual structural gene mutants defective in alginate synthesis.
Nucleoside diphosphate kinase: role in bacterial growth, virulence, cell signalling and polysaccharide synthesis
Specific examples of the role of Ndk in regulating growth, NTP formation and cell surface polysaccharide synthesis in two respiratory tract pathogens, Pseudomonas aeruginosa and Mycobacterium tuberculosis, are discussed.
Bacterial redox protein azurin, tumor suppressor protein p53, and regression of cancer
The use of a purified bacterial redox protein, azurin, that enters human cancer (melanoma UISO-Mel-2) cells and induces apoptosis is reported and has been shown to allow regression of human U ISO- Mel-2 tumors xenotransplanted in nude mice and may potentially be used in cancer treatment.
Characterization and regulation of the Pseudomonas aeruginosa algC gene encoding phosphomannomutase.
DNA sequence analysis of a defective algC gene (algC') cloned from a mutant of P. aeruginosa that lacked PMM activity revealed one point mutation (a C to T transition) in the carboxyl terminus of PMM which resulted in an amino acid change from arginine 420 to cysteine 420.
Chromate resistance plasmid in Pseudomonas fluorescens
Chromate resistance of Pseudomonas fluorescens LB300, isolated from chromium-contaminated sediment in the upper Hudson River, was found to be plasmid specified, and transformation of such strains with purified pLHB1 plasmids DNA resulted in a simultaneous re-acquisition of the chromate resistance phenotype and theplasmid.
Genetic mapping of chromosomal determinants for the production of the exopolysaccharide alginate in a Pseudomonas aeruginosa cystic fibrosis isolate
Evidence obtained indicated that the alg genes which undergo frequent mutation are chromosomal, that several loci are involved, and that these alg loci is apparently clustered on the chromosome.