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Preparative vertical and rotating horizontal (Rotofor) ampholine column and immobiline flat bed polyacrylamide gel isoelectric focusing were evaluated for the isolation of the biologically active… (More)
Desaturation of oleoyl CoA by the microsomal fraction of Fusarium oxysporum hyphal cells required O2, NADPH, MgCl2, and the addition of either bovine serum albumin or the 105 000g supernatant… (More)
The characteristics of the microsomal stearoyl CoA desaturase (EC 220.127.116.11) of vegetative Fusarium oxysporum cells grown at different temperatures were studied. The enzyme had an unusual preference… (More)
Suspension cultures of soybean cells incorporated [1-14C] acetate very rapidly into the fatty acid moieties of phospholipids and glycolipids when incubated at 26 C for up to 22 hr. The most rapidly… (More)
Abstract 1. 1. The surviving proteins in an 8-year-old sample of dried human blood were examined by physical and chemical techniques and shown to be modified. 2. 2. Ultracentrifugation and gel… (More)
Abstract 1. 1. A sample of whole human blood that had been stored in the dried state for 8 years at room temperature was tested for the presence of eleven specific globular proteins. 2. 2. Eight of… (More)
Abstract O -(Diphenylphosphinyl)hydroxylamine efficiently aminates a variety of stabilised carbanions and certain Grignard reagents.
In the Lowry method for protein, 1 mg of p -aminohippurate gives a color equivalent to 0.6 mg of protein. This method should not be used on fluids from patients receiving p -aminohippurate.