WDR5 Associates with Histone H3 Methylated at K4 and Is Essential for H3 K4 Methylation and Vertebrate Development
Brassinosteroid signal transduction from cell-surface receptor kinases to nuclear transcription factors
A complete BR signalling pathway is described by elucidating key missing steps and new insights are provided into the mechanism of GSK3 regulation.
BSKs Mediate Signal Transduction from the Receptor Kinase BRI1 in Arabidopsis
Genetic and transgenic studies demonstrate that BSKs are the substrates of BRI1 kinase that activate downstream BR signal transduction.
The CDG1 kinase mediates brassinosteroid signal transduction from BRI1 receptor kinase to BSU1 phosphatase and GSK3-like kinase BIN2.
Liquid droplet formation by HP1α suggests a role for phase separation in heterochromatin
It is shown by direct protein delivery into mammalian cells that an HP1 α mutant incapable of phase separation in vitro forms smaller and fewer nuclear puncta than phosphorylated HP1α, suggesting that heterochromatin-mediated gene silencing may occur in part through sequestration of compacted chromatin in phase-separated HP1 droplets, which are dissolved or formed by specific ligands on the basis of nuclear context.
Global landscape of HIV–human protein complexes
The use of affinity tagging and purification mass spectrometry is reported to determine systematically the physical interactions of all 18 HIV-1 proteins and polyproteins with host proteins in two different human cell lines (HEK293 and Jurkat).
Role of accurate mass measurement (+/- 10 ppm) in protein identification strategies employing MS or MS/MS and database searching.
This work explores the advantage of using accurate mass measurement (and thus constraint on the possible elemental composition of components in a protein digest) in strategies for searching protein, gene, and EST databases that employ mass values alone, fragment-ion tagging derived from MS/MS spectra, and de novo interpretation of MS/ MS spectra.
A Transmembrane Accessory Subunit that Modulates Kainate-Type Glutamate Receptors
Structural identification of autoinducer of Photobacterium fischeri luciferase.
- A. Eberhard, A. Burlingame, C. Eberhard, G. L. Kenyon, K. Nealson, N. Oppenheimer
- 28 April 1981
Synthesis of bacterial luciferase in some strains of luminous bacteria requires a threshold concentration of an autoinducer synthesized by the bacteria and excreted into the medium. Autoinducer…
Global Identification and Characterization of Both O-GlcNAcylation and Phosphorylation at the Murine Synapse*
Over 1750 and 16,500 sites of O-GlcNAcylation and phosphorylation from murine synaptosomes, respectively are identified, indicating the potential for crosstalk of phosphorylated with O- GlcNACylation via regulation of enzymatic activity.