Maintenance of pluripotency in human and mouse embryonic stem cells through activation of Wnt signaling by a pharmacological GSK-3-specific inhibitor
It is shown that activation of the canonical Wnt pathway is sufficient to maintain self-renewal of both HESCs and MESCs, and the use of GSK-3-specific inhibitors such as BIO may have practical applications in regenerative medicine.
GSK-3-selective inhibitors derived from Tyrian purple indirubins.
WDR5 Associates with Histone H3 Methylated at K4 and Is Essential for H3 K4 Methylation and Vertebrate Development
In Vivo Imaging of Quantum Dots Encapsulated in Phospholipid Micelles
- B. Dubertret, P. Skourides, D. Norris, V. Noireaux, A. Brivanlou, A. Libchaber
- Biology, ChemistryScience
- 29 November 2002
C encapsulated individual nanocrystals in phospholipid block–copolymer micelles acted as in vitro fluorescent probes to hybridize to specific complementary sequences and were followed to the tadpole stage, allowing lineage-tracing experiments in embryogenesis.
Balancing BMP signaling through integrated inputs into the Smad1 linker.
Molecular signature of human embryonic stem cells and its comparison with the mouse.
The orphan receptor ALK7 and the Activin receptor ALK4 mediate signaling by Nodal proteins during vertebrate development.
Nodal proteins have crucial roles in mesendoderm formation and left-right patterning during vertebrate development. The molecular mechanisms of signal transduction by Nodal and related ligands,…
A method to recapitulate early embryonic spatial patterning in human embryonic stem cells
It is demonstrated that the intrinsic tendency of stem cells to make patterns can be harnessed by controlling colony geometries and provide a quantitative assay for studying paracrine signaling in early development.
Expression of an engrailed-related protein is induced in the anterior neural ectoderm of early Xenopus embryos.
A monoclonal antibody directed against the C-terminus of the Drosophila invected homeodomain is used to detect a nuclear protein in brain cells of Xenopus laevis embryos, which is referred to as the Xenopus EN protein.
Neural induction, the default model and embryonic stem cells
The role of fibroblast growth factors and Wnt proteins in neural induction and in the regulation of BMP signalling in the ectoderm of Xenopus laevis and chick embryos are discussed and evidence from mouse embryonic stem cells that supports the default model of neural induction is discussed.