• Publications
  • Influence
Lipoxygenases: Occurrence, Functions, Catalysis, and Acquisition of Substrate*
  • A. Brash
  • Chemistry, Medicine
  • The Journal of Biological Chemistry
  • 20 August 1999
TLDR
In this review the diversity of lipoxygenase expression will be highlighted and the several facets of lip oxygengenase function considered, concluding with a discussion of issues related to the acquisition of substrate in a cellular environment. Expand
Arachidonic acid as a bioactive molecule.
  • A. Brash
  • Chemistry, Medicine
  • The Journal of clinical investigation
  • 1 June 2001
TLDR
The solubility properties of the molecule are considered, which are crucial to understanding the availability within the cell of endogenous and exogenous arachidonic acid, and selected biological actions of this lipid are turned to. Expand
The Structure of Human 5-Lipoxygenase
TLDR
The crystal structure at 2.4 angstrom resolution of human 5-LOX stabilized by replacement of this sequence that is involved in orienting the carboxyl terminus, which binds the catalytic iron. Expand
Discovery of a second 15S-lipoxygenase in humans.
TLDR
The detected and characterized a previously unrecognized enzyme that at least partly accounts for the 15S-lipoxygenase metabolism of arachidonic acid in certain epithelial tissues, and the different catalytic activities and tissue distribution suggest a distinct function for the new enzyme compared with the previously reported human 15S. Expand
A single active site residue directs oxygenation stereospecificity in lipoxygenases: stereocontrol is linked to the position of oxygenation.
  • G. Coffa, A. Brash
  • Chemistry, Medicine
  • Proceedings of the National Academy of Sciences…
  • 2 November 2004
TLDR
A model of lipoxygenase reaction specificity is proposed in which product regiochemistry and stereochemistry are determined by fixed relationships between substrate orientation, hydrogen abstraction, and the Gly or Ala residue the authors have identified. Expand
Purification of an allene oxide synthase and identification of the enzyme as a cytochrome P-450.
TLDR
The results establish a new catalytic activity for a cytochrome P-450 and illustrate the cooperation of different oxygenases in pathways of fatty acid metabolism. Expand
Control of oxygenation in lipoxygenase and cyclooxygenase catalysis.
TLDR
Four mechanistic models, not mutually exclusive, that could account for the specific reactions of molecular oxygen with a fatty acid in the LOX or COX active site are presented. Expand
15-Lipoxygenase Metabolism of 2-Arachidonylglycerol
TLDR
It is demonstrated that 15-LOXs are capable of acting on 2-AG to provide 15-HETE-G and elucidate a potential role for endocannabinoid oxygenation in the generation of peroxisome proliferator-activated receptor α agonists. Expand
Endogenous biosynthesis of prostacyclin and thromboxane and platelet function during chronic administration of aspirin in man.
TLDR
Although chronic administration of aspirin results in inhibition of endogenous throm boxane and prostacyclin biosynthesis over a wide dose range, inhibition of thromboxane biosynthesis is more selective at 20 than at 2,600 mg aspirin/d, however, despite this, inhibited platelet function is not maximal at the lower aspirin dosage. Expand
Two Distinct Pathways of Formation of 4-Hydroxynonenal
TLDR
Using the 9- and 13-hydroperoxides of linoleic acid as starting material, it is found that two distinct mechanisms lead to the formation of 4-H(P)NE and the corresponding 4-Hydro(pero)xyalkenal that retains the original carboxyl group. Expand
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