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The biosynthetic gene cluster for the antitumor rebeccamycin: characterization and generation of indolocarbazole derivatives.
Activation and identification of five clusters for secondary metabolites in Streptomyces albus J1074
A cluster for glycosylated compounds (paulomycins) was also identified by comparison of the high‐performance liquid chromatography profiles of the wild‐type strain with that of a mutant in which two key enzymes of the cluster were simultaneously deleted.
Reevaluation of the Violacein Biosynthetic Pathway and its Relationship to Indolocarbazole Biosynthesis
- C. Sánchez, A. Braña, C. Méndez, J. Salas
- Biology, ChemistryChembiochem : a European journal of chemical…
- 4 August 2006
The violacein pathway is studied through expression of vio genes in Escherichia coli and Streptomyces albus and a pair of genes (vioAB) was functionally equivalent to the homologous pair in the indolocarbazole pathway (rebOD), directing the formation of chromopyrrolic acid.
Combinatorial biosynthesis of antitumor indolocarbazole compounds.
- C. Sánchez, Lili Zhu, J. Salas
- Biology, ChemistryProceedings of the National Academy of Sciences…
- 11 January 2005
A biological process based on combinatorial biosynthesis for the production of indolocarbazole compounds (or their precursors) in engineered microorganisms as a complementary approach to chemical synthesis is reported.
Mithramycin SK, a novel antitumor drug with improved therapeutic index, mithramycin SA, and demycarosyl-mithramycin SK: three new products generated in the mithramycin producer Streptomyces…
- L. Remsing, A. González, J. Rohr
- Biology, ChemistryJournal of the American Chemical Society
- 14 May 2003
The structures of these three compounds confirmed indirectly the proposed role of MtmW in MTM biosynthesis, and the new mithramycin derivatives bear unexpectedly shorter 3-side chains than MTM, presumably caused by nonenzymatic rearrangement or cleavage reactions of the initially formed pentyl side chain with a reactive beta-dicarbonyl functional group.
Deciphering biosynthesis of the RNA polymerase inhibitor streptolydigin and generation of glycosylated derivatives.
Identification of Two Genes from Streptomyces argillaceus Encoding Glycosyltransferases Involved in Transfer of a Disaccharide during Biosynthesis of the Antitumor Drug Mithramycin
It is proposed that the glycosyltransferases encoded by mtmGI and mtmGII are responsible for forming and transferring the disaccharide during mithramycin biosynthesis.
Deciphering the late steps in the biosynthesis of the anti‐tumour indolocarbazole staurosporine: sugar donor substrate flexibility of the StaG glycosyltransferase
Staurosporine biosynthesis was reconstituted in vivo in a heterologous host Streptomyces albus by using two different plasmids: the ‘aglycone vector’ expressing a set of genes involved in indolocarbazole biosynthesis together with staG (encoding a glycosyltransferase) and/or staN (coding for a P450 oxygenase).
The Mithramycin Gene Cluster of Streptomyces argillaceus Contains a Positive Regulatory Gene and Two Repeated DNA Sequences That Are Located at Both Ends of the Cluster
The mtmR gene restored actinorhodin production in Streptomyces coelicolor JF1 mutant, in which the actin orhodin-specific activator ActII-orf4 is inactive, and also stimulated actinors production by Streptomeces lividans TK21.
Pathways and Regulation of Ammonium Assimilation in Streptomyces clavuligerus
The pattern of utilization of nitrogen sources by the three classes of mutants indicated that the glutamine synthetase–glutamate synthase pathway is the only means of assimilation of ammonium in S. clavuligerus.