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Generation of transgenic potato plants highly resistant to potato virus Y (PVY) through RNA silencing
In this study we applied RNA silencing to engineer potato plants that are resistant to potato virus Y (PVY). We expressed double-stranded (ds) RNA derived from the 3′ terminal part of the coatExpand
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Short 5′-phosphorylated double-stranded RNAs induce RNA interference in Drosophila
Double-stranded (ds) RNA causes the specific degradation of homologous RNAs in a process called "RNA interference (RNAi)"[1-4]; this process is called "posttranscriptional gene silencing (PTGS)" inExpand
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Prediction of novel microRNA genes in cancer-associated genomic regions—a combined computational and experimental approach
The majority of existing computational tools rely on sequence homology and/or structural similarity to identify novel microRNA (miRNA) genes. Recently supervised algorithms are utilized to addressExpand
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Developmental defects by antisense-mediated inactivation of micro-RNAs 2 and 13 in Drosophila and the identification of putative target genes.
Micro-RNAs are a class of small non-coding regulatory RNAs that impair translation by imperfect base pairing to mRNAs. For analysis of their cellular function we injected different miRNA-specific DNAExpand
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Short interfering RNAs specific for potato spindle tuber viroid are found in the cytoplasm but not in the nucleus.
Short interfering (si) and micro (mi) RNAs influence gene expression at post-transcriptional level. In plants, different classes of DICER-LIKE (DCL) enzymes are responsible for the generation ofExpand
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Variations of the 3' protruding ends in synthetic short interfering RNA (siRNA) tested by microinjection in Drosophila embryos.
Short interfering RNAs (siRNAs) are the processing product originating from long double-stranded RNAs (dsRNAs) that are cleaved by the RNase III-like ribonuclease Dicer. As siRNAs mediate cleavage ofExpand
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Induction of RNA interference in Caenorhabditis elegans by RNAs derived from plants exhibiting post-transcriptional gene silencing.
The term 'gene silencing' refers to transcriptional and post-transcriptional control of gene expression. Related processes are found across kingdoms in plants and animals. We intended to test whetherExpand
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The second cysteine‐rich domain of Mac1p is a potent transactivator that modulates DNA binding efficiency and functionality of the protein
Mac1p is a Saccharomyces cerevisiae DNA binding transcription factor that activates genes involved in copper uptake. A copper‐induced N–C‐terminal intramolecular interaction and copper‐independentExpand
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Experimental Strategies for the Identification and Validation of Target RNAs that Are Regulated by miRNAs
Micro-RNAs (miRNAs) are single-stranded RNA molecules of about 20 nucleotides and represent a class of small non-coding regulatory RNAs found in higher eukaryotes across kingdoms. miRNAs originateExpand