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Carbohydrate-binding modules: fine-tuning polysaccharide recognition.
TLDR
The present review summarizes the impact structural biology has had on the understanding of the mechanisms by which CBMs bind to their target ligands. Expand
Understanding the Biological Rationale for the Diversity of Cellulose-directed Carbohydrate-binding Modules in Prokaryotic Enzymes*
TLDR
The differential recognition of cell walls of diverse origin provides a biological rationale for the diversity of cellulose-directed CBMs that occur in cell wall hydrolases and conversely reveals the variety of cellulOSE microstructures in primary and secondary cell walls. Expand
Restricted access of proteins to mannan polysaccharides in intact plant cell walls.
TLDR
It is shown that molecular recognition of mannan polysaccharides present in intact cell walls is severely restricted and that the masking of primary cell wall mannan by pectin is a potential mechanism for controlling cell wall micro-environments. Expand
The biochemical properties of the Francisella pathogenicity island (FPI)-encoded proteins IglA, IglB, IglC, PdpB and DotU suggest roles in type VI secretion.
TLDR
The results may provide a mechanistic basis for many of the studies that have examined the virulence properties of Francisella mutants in FPI genes, namely that the observed phenotypes of the mutants are the result of the disruption of the FPI-encoded T6SS structure. Expand
Advances in understanding the molecular basis of plant cell wall polysaccharide recognition by carbohydrate-binding modules.
TLDR
A refinement to the Types A, B, and C classification of CBMs is proposed whereby the Type A CBMs remain those that bind the surfaces of crystalline polysaccharides but the Type B CBMs are redefined as those thatbind internally on glycan chains (endo-type), and CBMs that bind to the termini of gly can chains are defined as Type C modules (exo-type). Expand
Human gut Bacteroidetes can utilize yeast mannan through a selfish mechanism
TLDR
Genomic comparison with B. thetaiotaomicron in conjunction with cell culture studies show that a cohort of highly successful members of the microbiota has evolved to consume sterically-restricted yeast glycans, an adaptation that may reflect the incorporation of eukaryotic microorganisms into the human diet. Expand
Carbohydrate recognition by a large sialidase toxin from Clostridium perfringens.
TLDR
The results indicate that NanJ contains carbohydrate specific binding modules that likely function to target the enzyme to molecules or cells bearing mixed populations of glycans that terminate in either galactose/N-acetylgalactosamine or sialic acid. Expand
Characterization and affinity applications of cellulose-binding domains.
TLDR
CBDs with affinity for crystalline cellulose are useful tags for classical column affinity chromatography and the affinity of CBD(N1) for soluble cellulosics makes it suitable for use in large-scale aqueous two-phase affinity partitioning systems. Expand
Structural Biology of Pectin Degradation by Enterobacteriaceae
TLDR
A structural understanding of the molecular determinants of pectin utilization and the mechanisms driving catabolite selectivity and flow through the pathway is provided. Expand
Carbohydrate-binding Modules Recognize Fine Substructures of Cellulose*
TLDR
The demonstration of the very fine binding specificity of cellulose-specific CBMs implies that the polysaccharide targets of CBMs extend down to the resolution of cellulOSE microstructures. Expand
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