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Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting
TALENs are important new tools for genome engineering. Fusions of transcription activator-like (TAL) effectors of plant pathogenic Xanthomonas spp. to the FokI nuclease, TALENs bind and cleave DNA inExpand
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A Simple Cipher Governs DNA Recognition by TAL Effectors
TAL Order Xanthomonas bacteria attack their plant hosts by delivering their own transcription-activator–like (TAL) proteins into the plant cell nucleus and alter the plant's gene regulation (see theExpand
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TAL Effector-Nucleotide Targeter (TALE-NT) 2.0: tools for TAL effector design and target prediction
We have developed a suite of web-based tools called TAL Effector-Nucleotide Targeter 2.0 (TALE-NT2.0; https://boglab.iastate.edu/) that enables design of custom TAL effector repeat arrays for desired targets. Expand
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Targeting DNA Double-Strand Breaks with TAL Effector Nucleases
Engineered nucleases that cleave specific DNA sequences in vivo are valuable reagents for targeted mutagenesis. Here we report a new class of sequence-specific nucleases created by fusingExpand
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Understanding the functions of plant disease resistance proteins.
Many disease resistance (R) proteins of plants detect the presence of disease-causing bacteria, viruses, or fungi by recognizing specific pathogen effector molecules that are produced during theExpand
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Xanthomonas oryzae pathovars: model pathogens of a model crop.
SUMMARY Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola cause bacterial blight and bacterial leaf streak of rice (Oryza sativa), which constrain production of this staple crop inExpand
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Transcription Activator-Like Effector Nucleases Enable Efficient Plant Genome Engineering1[W][OA]
The ability to precisely engineer plant genomes offers much potential for advancing basic and applied plant biology. Here, we describe methods for the targeted modification of plant genomes usingExpand
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The Crystal Structure of TAL Effector PthXo1 Bound to Its DNA Target
Wrapped DNA TAL effectors are proteins that bacterial pathogens inject into plant cells that bind to host DNA to activate expression of plant genes. The DNA-binding domain of TAL proteins is composedExpand
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TAL Effectors: Customizable Proteins for DNA Targeting
Generating and applying new knowledge from the wealth of available genomic information is hindered, in part, by the difficulty of altering nucleotide sequences and expression of genes in living cellsExpand
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Pathogenomics of Xanthomonas: understanding bacterium–plant interactions
Xanthomonas is a large genus of Gram-negative bacteria that cause disease in hundreds of plant hosts, including many economically important crops. Pathogenic species and pathovars within species showExpand
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