Author pages are created from data sourced from our academic publisher partnerships and public sources.
Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting
TALENs are important new tools for genome engineering. Fusions of transcription activator-like (TAL) effectors of plant pathogenic Xanthomonas spp. to the FokI nuclease, TALENs bind and cleave DNA in… Expand
A Simple Cipher Governs DNA Recognition by TAL Effectors
TAL Order Xanthomonas bacteria attack their plant hosts by delivering their own transcription-activator–like (TAL) proteins into the plant cell nucleus and alter the plant's gene regulation (see the… Expand
TAL Effector-Nucleotide Targeter (TALE-NT) 2.0: tools for TAL effector design and target prediction
- Erin L. Doyle, Nicholas J. Booher, +4 authors A. Bogdanove
- Biology, Computer Science
- Nucleic Acids Res.
- 12 June 2012
We have developed a suite of web-based tools called TAL Effector-Nucleotide Targeter 2.0 (TALE-NT2.0; https://boglab.iastate.edu/) that enables design of custom TAL effector repeat arrays for desired targets. Expand
Targeting DNA Double-Strand Breaks with TAL Effector Nucleases
Engineered nucleases that cleave specific DNA sequences in vivo are valuable reagents for targeted mutagenesis. Here we report a new class of sequence-specific nucleases created by fusing… Expand
Understanding the functions of plant disease resistance proteins.
Many disease resistance (R) proteins of plants detect the presence of disease-causing bacteria, viruses, or fungi by recognizing specific pathogen effector molecules that are produced during the… Expand
Xanthomonas oryzae pathovars: model pathogens of a model crop.
SUMMARY Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola cause bacterial blight and bacterial leaf streak of rice (Oryza sativa), which constrain production of this staple crop in… Expand
Transcription Activator-Like Effector Nucleases Enable Efficient Plant Genome Engineering1[W][OA]
The ability to precisely engineer plant genomes offers much potential for advancing basic and applied plant biology. Here, we describe methods for the targeted modification of plant genomes using… Expand
The Crystal Structure of TAL Effector PthXo1 Bound to Its DNA Target
- Amanda Nga-Sze Mak, Philip Bradley, R. A. Cernadas, A. Bogdanove, B. Stoddard
- Biology, Medicine
- 10 February 2012
Wrapped DNA TAL effectors are proteins that bacterial pathogens inject into plant cells that bind to host DNA to activate expression of plant genes. The DNA-binding domain of TAL proteins is composed… Expand
TAL Effectors: Customizable Proteins for DNA Targeting
Generating and applying new knowledge from the wealth of available genomic information is hindered, in part, by the difficulty of altering nucleotide sequences and expression of genes in living cells… Expand
Pathogenomics of Xanthomonas: understanding bacterium–plant interactions
- R. Ryan, Frank-Jörg Vorhölter, +4 authors J. Dow
- Biology, Medicine
- Nature Reviews Microbiology
- 1 May 2011
Xanthomonas is a large genus of Gram-negative bacteria that cause disease in hundreds of plant hosts, including many economically important crops. Pathogenic species and pathovars within species show… Expand