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Isolation of an autotrophic ammonia-oxidizing marine archaeon
The isolation of a marine crenarchaeote that grows chemolithoautotrophically by aerobically oxidizing ammonia to nitrite—the first observation of nitrification in the Archaea is reported, suggesting that nitrifying marine Cren archaeota may be important to global carbon and nitrogen cycles. Expand
A PCR Assay To Discriminate Human and Ruminant Feces on the Basis of Host Differences in Bacteroides-Prevotella Genes Encoding 16S rRNA
This research recovered 16S rDNA clones from natural waters that were close phylogenetic relatives of the markers, and designed specific PCR primers that discriminate human and ruminant sources of fecal contamination. Expand
Identification of Nonpoint Sources of Fecal Pollution in Coastal Waters by Using Host-Specific 16S Ribosomal DNA Genetic Markers from Fecal Anaerobes
A new PCR-based method for distinguishing human and cow fecal contamination in coastal waters without culturing indicator organisms is described and it is shown that the method can be used to track bacterial marker sequences in complex environments. Expand
Host Distributions of Uncultivated Fecal Bacteroidales Bacteria Reveal Genetic Markers for Fecal Source Identification
Examination of host distribution patterns among fecal bacteria in the order Bacteroidales revealed both endemic and cosmopolitan distributions among the eight hosts, with the goal of using endemic sequences as markers for fecal source identification in aquatic environments. Expand
Loss of diversity of ammonia-oxidizing bacteria correlates with increasing salinity in an estuary system.
It is suggested that salinity is a strong environmental control on AOB diversity and distribution in this estuary. Expand
Distribution and Diversity of Archaeal and Bacterial Ammonia Oxidizers in Salt Marsh Sediments
The results expand the distribution of AOA to salt marshes, and the high numbers of Aoa at some sites suggest that salt marsh sediments serve as an important habitat for AOA, which was always more abundant than β-AOB based on quantitative PCR of amoA genes. Expand
Abundance of Ammonia-Oxidizing Archaea and Bacteria along an Estuarine Salinity Gradient in Relation to Potential Nitrification Rates
ABSTRACT Abundance of ammonia-oxidizing Archaea (AOA) was found to be always greater than that of ammonia-oxidizing Bacteria along an estuarine salinity gradient, and AOA abundance was highest atExpand
Functionally distinct communities of ammonia-oxidizing bacteria along an estuarine salinity gradient.
Although salinity appears to be an important factor in determining AOB distribution, it may not be the primary factor as AOB exhibited a broad range of salinity tolerance in the authors' experiments. Expand
Estuarine Nitrifiers: New Players, Patterns and Processes
Estuaries may serve as important natural laboratories in which to investigate the relationships between archaeal and bacterial ammonia oxidizers, and the data suggest that the variability in the observed patterns is likely not due to a simple salinity effect. Expand
Molecular approaches to microbiological monitoring: fecal source detection.
This approach is more sensitive than fecal coliform assays, is comparable in complexity to standard food safety and public health diagnostic tests, and lends itself to automation and high-throughput. Expand