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Structures of active conformations of Gi alpha 1 and the mechanism of GTP hydrolysis.
TLDR
AlF4- complexes formed by the G protein Gi alpha 1 demonstrate specific roles in transition-state stabilization for two highly conserved residues, suggesting a mechanism that may promote release of the beta gamma subunit complex when the alpha subunit is activated by GTP. Expand
Structure of the first C2 domain of synaptotagmin I: A novel Ca2+/phospholipid-binding fold
TLDR
The three-dimensional structure of Synaptotagmin I, a synaptic vesicle protein involved in the Ca2+ regulation of exocytosis, contains two C2 domains, the first of which acts as a Ca 2+ sensor. Expand
IRF8 mutations and human dendritic-cell immunodeficiency.
TLDR
These findings define a class of human primary immunodeficiencies that affect the differentiation of mononuclear phagocytes and show that human IRF8 is critical for the development of monocytes and dendritic cells and for antimycobacterial immunity. Expand
Crystal structures of cyclohexanone monooxygenase reveal complex domain movements and a sliding cofactor.
TLDR
The atomic structures of CHMO are presented from an environmental Rhodococcus strain bound with FAD and NADP(+) in two distinct states, to resolutions of 2.3 and 2.2 A. Taken together, these structures provide mechanistic insights into CHMO-catalyzed Baeyer-Villiger oxidation. Expand
YjeQ, an essential, conserved, uncharacterized protein from Escherichia coli, is an unusual GTPase with circularly permuted G-motifs and marked burst kinetics.
TLDR
E. coli YjeQ is an unusual, circularly permuted P-loop-containing GTPase, which catalyzes GTP hydrolysis at a rate 45 000 times greater than that of turnover. Expand
Crystal Structure of WbpP, a Genuine UDP-N-acetylglucosamine 4-Epimerase from Pseudomonas aeruginosa
TLDR
The examination indicates that as little as one residue can dictate substrate specificity, and phylogenetic analysis suggests that this substrate specificity is an evolutionary and highly conserved property within UDP-hexose 4-epimerases. Expand
Structure of an Enzyme Required for Aminoglycoside Antibiotic Resistance Reveals Homology to Eukaryotic Protein Kinases
TLDR
The three-dimensional fold of APH(3')-IIIa reveals a striking similarity to eukaryotic protein kinases despite a virtually complete lack of sequence homology, indicating that APH (3') enzymes and eukARYotic-typeprotein kinases share a common ancestor. Expand
A recurrent PDGFRB mutation causes familial infantile myofibromatosis.
TLDR
Findings indicate p.Arg561Cys substitution in PDGFR-β as a cause of the dominant form of this disease and provide a rationale for further investigations of this specific mutation and gene to assess the benefits of targeted therapies against PDG FR-β in aggressive life-threatening familial forms of the disease. Expand
Tertiary and quaternary structural changes in Gi alpha 1 induced by GTP hydrolysis.
TLDR
The amino and carboxyl termini, which contain receptor and beta gamma binding determinants, are disordered in the complex with GTP, but are organized into a compact microdomain on GDP hydrolysis, suggesting that multimers of alpha subunits or heterotrimers may play a role in signal transduction. Expand
Germline and somatic FGFR1 abnormalities in dysembryoplastic neuroepithelial tumors
TLDR
Constitutional and somatic FGFR1 alterations and MAP kinase pathway activation are key events in the pathogenesis of DNET and point the way towards existing targeted therapies. Expand
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