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Inhibition of Schistosoma mansoni Thioredoxin-glutathione Reductase by Auranofin
TLDR
It is proposed that Sec mediates the transfer of gold from its ligands in AF to the redox-active Cys couples of TGR, and can be mimicked by an external source of selenium (benzeneselenol).
Demonstration of long-range interactions in a PDZ domain by NMR, kinetics, and protein engineering.
TLDR
Structural and dynamical evidence for allosteric effects in a PDZ domain, PDZ2 from the tyrosine phosphatase PTP-BL, upon binding to a target peptide is presented and it is revealed that binding is regulated by long-range interactions, which can be correlated with the structural rearrangements resulting from peptide binding.
Glutathione reductase and thioredoxin reductase at the crossroad: The structure of Schistosoma mansoni thioredoxin glutathione reductase
TLDR
The crystal structure of TGR from Schistosoma mansoni is reported, deleted in the last two residues, and the peculiar architecture of this chimeric enzyme is revealed, updating the interpretation of the interdomain communication in TGR enzymes.
CO‐MP4, a polyethylene glycol‐conjugated haemoglobin derivative and carbon monoxide carrier that reduces myocardial infarct size in rats
TLDR
The functional interaction between MP4 and carbon monoxide (CO), as a CO delivery agent, and the effects of CO‐MP4 on myocardial infarct size following ischaemia and reperfusion in rats are described.
Switching between the alternative structures and functions of a 2-Cys peroxiredoxin, by site-directed mutagenesis.
TLDR
The structural events responsible for the moonlighting behavior of 2-Cys Prx are defined and it is demonstrated that acidification is coupled to local structural variations localized at the active site and a change in oligomerization to HMW forms, similar to those induced by oxidative stress.
Schistosoma mansoni fatty acid binding protein: specificity and functional control as revealed by crystallographic structure.
TLDR
Comparison with homologous fatty acid binding proteins suggests that the binding site of Sm14 is optimized to fit arachidonic acid, which is a vaccine target for schistosomiasis and proposed to have physiological relevance.
Evidence for two oxygen-linked binding sites for polyanions in dromedary hemoglobin.
TLDR
The pronounced sensitivity to solvent composition of the functional properties of dromedary hemoglobin even in the oxy state stresses the potential relevance of this conformation for regulating the oxygen transport in vivo.
Moonlighting by different stressors: crystal structure of the chaperone species of a 2-Cys peroxiredoxin.
TLDR
This work presents the structures of Schistosoma mansoni PrxI in both states: the LMW decamer and the HMW 20-mer formed by two stacked decamers, and proposes a model to account for the in vivo formation of long filaments of stacked Prx rings.
Fast-reacting Thiols in Rat Hemoglobins Can Intercept Damaging Species in Erythrocytes More Efficiently Than Glutathione*
TLDR
FHbSHs are expected to intercept damaging species in erythrocytes more efficiently than glutathione, thus adding a new physiopathological role (direct involvement in cellular strategies of antioxidant defense) to cysteinyl residues in proteins.
Mapping the Catalytic Cycle of Schistosoma mansoni Thioredoxin Glutathione Reductase by X-ray Crystallography*
TLDR
This study dissects the catalytic cycle of Schistosoma mansoni TGR by structural and functional analysis of the U597C mutant and proposes the putative functionally relevant conformational change of the C terminus after the transfer of reducing equivalents from NADPH to the redox sites of the enzyme.
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