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Complete nucleotide sequence of a putative new cytorhabdovirus infecting lettuce
The results indicate that this virus may be a member of a new cytorhabdovirus species, for which the name Lettuce yellow mottle virus (LYMoV) is proposed.
Delivery of Hairpin RNAs and Small RNAs Into Woody and Herbaceous Plants by Trunk Injection and Petiole Absorption
Simple methods for efficient delivery of hairpin RNAs and small interfering RNAs to Malus domestica, Vitis vinifera, and Nicotiana benthamiana that are based on trunk injection and/or petiole absorption are described and may have great impact in pest management against chewing and/ or xylem sap-feeding vectors and eukaryotic pathogens that reside in thexylem.
Mapping of the exchangeable and dispensable domains of the RNA 2-encoded 2A(HP) protein of arabis mosaic nepovirus.
Complete genome sequence of a virulent isolate of Arabis mosaic virus from privet (Ligustrum vulgare)
The complete RNA 1 and RNA 2 sequences of a virulent isolate of ArMV from privet (Ligustrum vulgare, Lv) are reported and sequence comparisons between this isolate and the corresponding sequences of other ArMv isolates and other nepoviruses are also reported.
Real-time multiplex RT-PCR for the simultaneous detection of the five main grapevine viruses.
High-Pressure-Sprayed Double Stranded RNA Does Not Induce RNA Interference of a Reporter Gene
Using a transgenic Nicotiana benthamiana line, it was found that high-pressure-sprayed naked dsRNA did not induce silencing of a green fluorescence protein (GFP) reporter gene, indicating that the ds RNA was insufficiently taken up by plant cells.
Characterization of proteinase cleavage sites in the N-terminal region of the RNA1-encoded polyprotein from Arabis mosaic virus (subgroup A nepovirus).
Short communication. Molecular analysis of the genomic RNAs 1 and 2 of the first Arabis mosaic virus isolate detected in Spanish grapevines
The nucleotide sequence of the genomic RNAs 1 and 2 of the first isolate of ArMV infecting grapevine detected in Spain (ArMV-DU13) has been determined and will be helpful to design more appropriate and reliable molecular diagnostic techniques for the control of this emerging virus in Spain.
In vitro and in vivo evidence for differences in the protease activity of two arabis mosaic nepovirus isolates and their impact on the infectivity of chimeric cDNA clones.
Replicating Potato spindle tuber viroid mediates de novo methylation of an intronic viroid sequence but no cleavage of the corresponding pre-mRNA
- Athanasios Dalakouras, E. Dadami, A. Bassler, Michèle Zwiebel, G. Krczal, M. Wassenegger
- BiologyRNA biology
- 4 March 2015
The data suggest that PSTVd fails to trigger post-transcriptional gene silencing (PTGS) and that RdDM and nuclear PTGS are not necessarily coupled.