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Determination of lymphocyte division by flow cytometry.
Techniques currently available for determining cell division are able to show one or, at best, a limited number of cell divisions. Other methods exist which can quantify overall division, but tellExpand
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Analysing cell division in vivo and in vitro using flow cytometric measurement of CFSE dye dilution.
  • A. B. Lyons
  • Biology, Medicine
  • Journal of immunological methods
  • 21 September 2000
Since its introduction in 1994 (J. Immunol. Methods 171 (1994) 131), the flow cytometric analysis of lymphocyte proliferation by serial halving of the fluorescence intensity of the vital dye CFSEExpand
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B cell differentiation and isotype switching is related to division cycle number
The mature, resting immunoglobulin (Ig) M, IgD+ B lymphocyte can be induced by T cells to proliferate, switch isotype, and differentiate into Ig-secreting or memory cells. Furthermore, B cellExpand
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Flow cytometric analysis of cell division history using dilution of carboxyfluorescein diacetate succinimidyl ester, a stably integrated fluorescent probe.
Publisher Summary This chapter reviews the use of a technique based on the serial dilution of a stably binding intracellular fluorochrome, carboxyfluorescein diacetate succinimidyl ester (CFSE),Expand
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Macrophage colony-stimulating factor receptor c-fms is a novel target of imatinib.
Imatinib is a tyrosine kinase inhibitor that suppresses the growth of bcr-abl-expressing chronic myeloid leukemia (CML) progenitor cells by blockade of the adenosine triphosphate (ATP)-binding siteExpand
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Cell division number regulates IgG1 and IgE switching of B cells following stimulation by CD40 ligand and IL‐4
CD40 ligand (CD40L) and IL‐4 are sufficient to induce resting murine B cells to divide and switch isotypes from IgM and IgD to IgG1 and IgE. Tracking of cell division following (5‐and 6)Expand
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A second transmissible cancer in Tasmanian devils
Significance Transmissible cancers are somatic cell lineages that are spread between individuals via the transfer of living cancer cells. Only three transmissible cancers have been reported inExpand
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The fate of self-reactive B cells depends primarily on the degree of antigen receptor engagement and availability of T cell help
Self-reactive B cells from tolerant double-transgenic (Dbl-Tg) mice coexpressing hen egg lysozyme (HEL) and rearranged anti-HEL immunoglobulin genes have a relatively short life span when compared toExpand
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Divided we stand: Tracking cell proliferation with carboxyfluorescein diacetate succinimidyl ester
  • A. B. Lyons
  • Biology, Medicine
  • Immunology and cell biology
  • 1 December 1999
Most techniques for assessing cell division can either detect limited numbers of cell divisions (bromodeoxyuridine incorporation) or only quantify overall proliferation (tritiated thymidineExpand
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Resistance to c-KIT kinase inhibitors conferred by V654A mutation
Certain mutations within c-KIT cause constitutive activation of the receptor and have been associated with several human malignancies. These include gastrointestinal stromal tumors (GIST),Expand
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