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Maturation of hydrogenases.
Enzymes possessing the capacity to oxidize molecular hydrogen have developed convergently three class of enzymes leading to: [FeFe]-, [NiFe]-, and [FeS]-cluster-free hydrogenases. They differ in theExpand
Mutational analysis of the operon (hyc) determining hydrogenase 3 formation in Escherichia coli
In‐frame deletions were introduced into each of the eight genes of the hyc operon coding for products required for the formation of the formate hydrogenlyase (FHL) system. The deletions wereExpand
Mechanism of regulation of the formate‐hydrogenlyase pathway by oxygen, nitrate, and pH: definition of the formate regulon
The products of a minimum of 15 genes are required for the synthesis of an active formate‐hydrogenlyase (FHL) system in Escherichia coli. All are co‐ordinately regulated in response to variations inExpand
On the mechanism of selenium tolerance in selenium-accumulating plants. Purification and characterization of a specific selenocysteine methyltransferase from cultured cells of Astragalus bisculatus.
Selected members of the genus Astragalus (Fabaceae) are known for their ability to accumulate high levels of selenium, mainly in the form of Se-methyl-selenocysteine. With the aid of cell cultures weExpand
Taming of a Poison: Biosynthesis of the NiFe-Hydrogenase Cyanide Ligands
NiFe-hydrogenases have an Ni-Fe site in which the iron has one CO and two CN groups as ligands. Synthesis of the CN ligands requires the activity of two hydrogenase maturation proteins: HypF andExpand
Selenocysteine: the 21st amino acid
Great excitement was elicited in the field of selenium biochemistry in 1986 by the parallel discoveries that the genes encoding the selenoproteins glutathione peroxidase and bacterial formateExpand
Desulfurolobus ambivalens, gen. nov., sp. nov., an autotrophic archaebacterium facultatively oxidizing or reducing sulfur
Summary The archaebacterium Desulfurolobus ambivalens gen. nov. sp. nov., family Sulfolobaceae , is an obligate chemolitho-autotroph, facultatively growing by the oxidation with O 2 or the reductionExpand
Selenocysteine tRNA‐specific elongation factor SelB is a structural chimaera of elongation and initiation factors
In all three kingdoms of life, SelB is a specialized translation elongation factor responsible for the cotranslational incorporation of selenocysteine into proteins by recoding of a UGA stop codon inExpand
Maturation of [NiFe]-hydrogenases in Escherichia coli: the HypC cycle.
Carbamoyl phosphate (CP) has been implicated as an educt for the synthesis of the CO and CN ligands of the metal centre of [NiFe]-hydrogenases in Escherichia coli, since CP synthetase mutants (carAB)Expand
S-methylmethionine metabolism in Escherichia coli.
Selenium-accumulating Astragalus spp. contain an enzyme which specifically transfers a methyl group from S-methylmethionine to the selenol of selenocysteine, thus converting it to a nontoxic, sinceExpand
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