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Structural evidence for regulation and specificity of flaviviral proteases and evolution of the Flaviviridae fold
The crystal structure of WNV NS2B‐NS3pro is reported both in a substrate‐free form and in complex with the trypsin inhibitor aprotinin/BPTI, providing evidence for an “induced fit” mechanism of catalysis and allowing the design of protease inhibitors to treat a range of flaviviral infections. Expand
Vaccinia virus N1L protein resembles a B cell lymphoma‐2 (Bcl‐2) family protein
The crystal structure of N1L is reported, which reveals an unexpected but striking resemblance to host apoptotic regulators of the B cell lymphoma‐2 (Bcl‐2) family, and it is shown that N1 l binds with high affinity to the BH3 peptides of pro‐apoptotic B cl‐2 family proteins in vitro, consistent with a role for N1l in modulating host antiviral defenses. Expand
Crystal structure of a glucose/H+ symporter and its mechanism of action
A mechanism for glucose/H+ symport is proposed and the symport mechanism versus facilitated diffusion is discussed, and the crystal structure of GlcPSe is reported, providing insight into the mechanism of glucose transport. Expand
Crystal structure and evolution of a prokaryotic glucoamylase.
Domains similar to those of prokaryotic glucoamylases in maltose phosphorylases and glycoaminoglycan lyases suggest evolution from a common ancestor. Expand
Structure of Complement C6 Suggests a Mechanism for Initiation and Unidirectional, Sequential Assembly of Membrane Attack Complex (MAC)*♦
The crystal structure of C6, the first and longest of the pore proteins to be recruited by C5b, is reported and a model of the assembled pore resembles those of the cholesterol-dependent cytolysins but is distinct from that recently proposed for perforin. Expand
Crystal structure of glucoamylase from Aspergillus awamori var. X100 to 2.2-A resolution.
The model developed for glucoamylase is a rare and valuable structural example of a glycoprotein and an exo-acting amylolytic enzyme. Expand
Crystal Structure of C5b-6 Suggests Structural Basis for Priming Assembly of the Membrane Attack Complex*
An atomic model of the C5b-6 complex, which triggers assembly of the Membrane Attack Complex, shows that C5B provides four interfaces for the auxiliary domains of C6, and suggests novel small molecule strategies for modulating pathological MAC assembly. Expand
NS4A regulates the ATPase activity of the NS3 helicase: a novel cofactor role of the non-structural protein NS4A from West Nile virus.
It is concluded that, similar to HCV NS4A,NS4A of WNV acts as a cofactor for NS3hel and allows helicase to sustain the unwinding rate of the viral RNA under conditions of ATP deficiency. Expand
Crystallographic complexes of glucoamylase with maltooligosaccharide analogs: relationship of stereochemical distortions at the nonreducing end to the catalytic mechanism.
The magnitude of stereochemical distortion observed in theactive site of glucoamylase suggests that favorable electrostatic interactions between the putative glucopyranosyl cation intermediate and the active site must be more important in stabilizing the transition state than mechanical distortion of the substrate. Expand
Refined structure for the complex of d‐gluco‐dihydroacarbose with glucoamylase from Aspergillus awamori var. X100 to 2.2 Å resolution: dual conformations for extended inhibitors bound to the active
The crystal structure at pH 4 of the complex of glucoamylase II(471) from Aspergillus awamori var. X100 with the pseudotetrasaccharide d‐gluco‐dihydroacarbose has been refined to an R‐factor of 0.125Expand