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Detection and quantification of alkaline phosphatase in single cells of phosphorus-starved marine phytoplankton
Alkaline phosphatase (AP) activity in marine and freshwater phytoplankton has been associated with phosphorus (P) limitation whereby the enzyme functions in the breakdown of exogenous organic PExpand
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Eukaryotic Organisms in Extreme Acidic Environments, the Río Tinto Case
A major issue in microbial ecology is to identify the limits of life for growth and survival, and to understand the molecular mechanisms that define these limits. Thus, interest in the biodiversityExpand
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Development and Structure of Eukaryotic Biofilms in an Extreme Acidic Environment, Río Tinto (SW, Spain)
An in situ colonization assay was performed to study the early stages of biofilm formation in Río Tinto (SW, Spain), an extremely acidic environment (pH ca. 2). Eukaryotic assemblages were monitoredExpand
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First Prokaryotic Biodiversity Assessment Using Molecular Techniques of an Acidic River in Neuquén, Argentina
Two acidic hot springs close to the crater of Copahue Volcano (Neuquén, Argentina) are the source of the Río Agrio. The river runs several kilometres before flowing into Caviahue Lake. Along theExpand
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The use of polyclonal antisera and blocking of antibodies in the identification of marine dinoflagellates: species-specific and clone-specific antisera against Gymnodinium and Alexandrium
Abstract Separate polyclonal antibodies were developed against cell surface antigens of the 4 dinoflagellate species: (a) Gymnodinium catenatum ; (b) a morphologically similar non-toxicExpand
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Immunomagnetic isolation of live and preserved Alexandrium fundyense cells
A method to isolate cells of the toxic dinoflagellate Alexandrium fundyense from sea- water samples using magnetic beads was evaluated to determine whether it could be used to obtain species-specificExpand
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Long-term results of limb salvage with the Fabroni custom made endoprosthesis.
In 1972 the authors began working on a limb amputation method using custom endoprostheses. Because of the biomechanical characteristics of these prostheses, good long-term results have been achieved.Expand
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Comparative Transcriptomic Analysis of the Response of Dunaliella acidophila (Chlorophyta) to Short-Term Cadmium and Chronic Natural Metal-Rich Water Exposures
Heavy metals are toxic compounds known to cause multiple and severe cellular damage. However, acidophilic extremophiles are able to cope with very high concentrations of heavy metals. This studyExpand
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Immunomagnetic separation of cells of the toxic dinoflagellate Alexandrium fundyense from natural plankton samples
A novel method was developed for isolating cells of the toxic dinoflagellate Alexandrium fundyense from preserved natural seawater samples using paramagnebc beads and a monoclonal antibody againstExpand
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