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Monoclonal Antibodies and the Identification of Functional Regions of the Na+,K+‐ATPase a
Na+,K+-ATPase (EC is the plasma membrane pump that maintains cellular electrochemical gradients of Na+ and K+ and the cardiac glycoside receptor. The enzyme has two subunits, the a subunitExpand
Structural dynamics and oligomeric interactions of Na+,K(+)-ATPase as monitored using fluorescence energy transfer.
The oligomeric nature of the purified lamb kidney Na+,K(+)-ATPase was investigated by measuring the fluorescence energy transfer between catalytic (alpha) subunits following sequential labeling withExpand
Immunochemical and spectroscopic characterization of two fluorescein 5'-isothiocyanate labeling sites on Na+,K(+)-ATPase.
Fluorescein 5'-isothiocyanate (FITC) covalently modifies the Lys-501 residue of the catalytic (alpha) subunit of Na+,K(+)-ATPase and resides at a conformation-sensitive site in or near the ATPExpand
The inhibitory monoclonal antibody M7-PB-E9 stabilizes E2 conformational states of Na+,K(+)-ATPase.
Monoclonal antibody M7-PB-E9 binds the sheep kidney Na+,K(+)-ATPase alpha-subunit with high affinity (Kd = 3 nM) and inhibits enzyme turnover in competition with ATP, and, like ATP, in the presenceExpand
The epitope for the inhibitory antibody M7-PB-E9 contains Ser-646 and Asp-652 of the sheep Na+,K(+)-ATPase alpha-subunit.
The binding of monoclonal antibody M7-PB-E9 to the alpha-subunit of Na+,K(+)-ATPase partially inhibits enzyme activity (35%) in competition with ATP, while in the presence of magnesium it stimulatesExpand
The carbohydrate moieties of the beta-subunit of Na+, K(+)-ATPase: their lateral motions and proximity to the cardiac glycoside site.
The beta-subunit associated with the catalytic (alpha) subunit of the mammalian Na+, K(+) -ATPase is a transmembrane glycoprotein with three extracellularly located N-glycosylation sites. AlthoughExpand