A. V. Razygraev

  • Citations Per Year
Learn More
Albumin molecules, unlike molecules of many other plasma proteins, are not covered with a carbohydrate shell. Albumin plays a crucial role in maintaining colloid osmotic blood pressure and can bind and transport various endogenous and exogenous molecules. The enzymatic activity of albumin, the existence and the role of which most researchers are still(More)
Glutathione peroxidase activity was studied in structures of the white and gray matter of adult female rats using H2O2 as a substrate and Ellman’s reagent to determine the decline in the level of the reduced glutathione. The maximum activity expressed in μmole GSH/min/mg protein was observed in the pyramids of the medulla oblongata (white matter) and the(More)
Using benzylamine as a substrate, the amine oxidase activity was determined in the pineal gland of adult rats and compared with the same activity in brain areas and pituitary. Two groups of rats aged 6-8 and 14-15 months were also compared on the basis of this activity. Benzylamine deaminating activity in the pineal gland was significantly higher than in(More)
Earlier it has been shown that extracellular glutathione peroxidase (GPx3) from human plasma is able to use cysteine (Cys-SH) instead of glutathione (GSH) as a thiol substrate. In the present study, the ability of rat plasma to utilize not only GSH, but also Cys-SH and homocysteine (Hcy-SH), in the thiol peroxidase reaction has been confirmed. The molar(More)
Total monoamine oxidase activity was investigated in the pineal gland (epiphysis) and in three brain structures with the use of spectrophotometric method based on kynuramine oxidative deamination, the product (4-hydroxyquinoline) formation being detected at 327 nm. Female Wistar rats used for the experiment were 1.5-2, 4-5 and over 12 months old. In the(More)
Total monoamine oxidase activity in the medial preoptic area and median eminence (with surrounding tissue) has been studied in female rats of three age groups, viz., those aged 1.5–2 months (peripubertal), 4–5 months (mature), and over 12 months (aging). Monoamine oxidase activity was measured using kynuramine as a substrate and changes in the concentration(More)
Spectrophotometric detection (at 327 nm) of a product of the kynuramine oxidation was used to study the distribution of the total activity of monoamine oxidase (MAO) in the hypothalamus, limbic system, dorsal raphe nucleus, locus coeruleus (with surrounding tissues), and hypophysis of female rats. The highest and lowest activities (8.03 ± 0.28 and 0.61 ±(More)
Glutathione peroxidase activity has been studied in the pineal gland (epiphysis) of young and aging female Wistar rats (2-4 and 17-19 month old). For comparison the same activity was studied in the pyramids of medulla oblongata and in the olfactory tubercle. These two brain structures represent white and gray matter respectively. The determination of the(More)
Early studies recognized that the human extracellular glutathione peroxidase may function as cysteine peroxidase (cysteine:H2O2 oxidoreductase). The aim of the present study was to test the hypothesis of cysteine:H2O2 oxidoreductase activity in rat plasma. The rat plasma was found to be able to accelerate greatly the H2O2-dependent oxidation of cysteine.(More)
Recently it was shown that the presence of rat blood plasma (as well as of erythrocyte hemolysate) in the reaction mixture containing 43 mM Tris-HCl-buffer (pH 8.5), 0.29 mM EDTA, 19.2 mM sodium azide, 1 mM DL-homocysteine (Hcy), and 198 mM hydrogen peroxide (incubation at 37 degrees C) results in a significant acceleration of the decrease in Hcy(More)