A V Iakh''iaev

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Nucleoside diphosphate (NDP) kinase from bovine retina was found to contain carbohydrates. The subunits of NDP kinase were separated by SDS-PAGE, blotted onto an Immobilon-P membrane, and their carbohydrate content was determined. Both subunits contained equal amounts of Gal, Man, Fuc, Gal-NAc, and Glc-NAc. The total carbohydrate content was 2 to 3% of the(More)
Using spin trapping method there were discovered and identified the radicals of linolenic acid formed when initiating its peroxidation by the system Fe2+-ascorbate. Mechanism of formation of linolenic acid radicals and their role in initiation of peroxidation were studied. A scheme of reactions of peroxidation initiation in the system Fe2+-ascorbate.(More)
The mechanism of free radical generation in the reaction of ferrous ion with t-butyl and linolenic acid hydroperoxide was investigated by spin trapping method. The t-butoxyl, methyl, linolenic acid alkoxyl and alkyl radical spin adducts EPR spectra were observed and identified.
An electrophoretically homogeneous preparation of tyrosinase (Mr = 61 kDa) was isolated from rat skin. The purification procedure which consisted in chromatographic separation of Triton X-100-solubilized proteins included four main steps, namely: gel filtration, anion-exchange chromatography and two consecutive affinity chromatography steps.(More)
Effect of alpha-melanocyte stimulating hormone (MSH) on proliferating activity of MS and BRO melanoma cell strains with dissimilar phenotype was studied. MSH, apart from the effect on melanomagenesis, influenced the cell proliferation. Growth of MS cells was inhibited at 10(-8)-10(-9) M concentrations of the hormone (IC50 = 2.10(-8) M), while BRO cells were(More)
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