A. Subramanian

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Chondrocytes, the cellular component of the articular cartilage, have long been recognized as strain-sensitive cells, and have the ability to sense mechanical stimulation through surface receptors and intracellular signaling pathways. This strain-induced biological response of chondrocytes has been exploited to facilitate chondrocyte culture in in vitro(More)
A theoretical analysis is presented with experimental confirmation to conclusively demonstrate the critical role that annealing plays in efficient PCR amplification of GC-rich templates. The analysis is focused on the annealing of primers at alternative binding sites (competitive annealing) and the main result is a quantitative expression of the efficiency(More)
Production of recombinant antibodies against botulinum neurotoxin is necessary for the development of a post-exposure treatment. CHO-DG44 cells were transfected with a plasmid encoding the light and heavy chains of a chimeric monoclonal antibody (S25) against botulism neurotoxin serotype A. Stable cell lines were obtained by dilution cloning and clones were(More)
Tissue engineering concepts and methodologies that employ biocompatible matrices or scaffolds have the potential to meet needs encountered in the repair of defects in articular cartilage. A desirable design parameter in the tissue engineering of cartilage in vitro is the development of seeded scaffolds with appropriate structure, composition, mechanical(More)
Humans demonstrate limited spontaneous endothelialization of prosthetic bypass grafts. However the local application of growth factors to prosthetic grafts or to injured blood vessels can provide an immediate effect on endothelialization. Novel chimeric proteins combining potent angiogens with extracellular matrix binding domains may localize to exposed(More)
A d'Alembert-based solution of forced wave motion with internal and boundary damping is presented with the specific intention of investigating the transient response. The dynamic boundary condition is a convenient method to model the absorption and reflection effects of an interface without considering coupled PDE's. Problems with boundary condition of the(More)
3 dimensional, porous, biodegradable scaffolds were fabricated using modified starch of varying degree of substitution (DS) by extrusion processing. Freeze drying/lyophilization was also employed to fabricate scaffolds from modified starch. The research efforts have been focused on the comparison of the above-mentioned techniques by comparing the properties(More)
A fully-digital, single-loop Unified Voltage and Frequency Regulator (UVFR) is designed in 130nm CMOS to provide the correct supply to digital loads to meet a timing criteria. Simultaneously a Tunable Replica Circuit (TRC) based local oscillator is generated from the regulated supply and clocks the load. Measurements show 0.84V to 0.27V range of operation,(More)
Zirconia particles modified with N,N,N',N'-ethylenediaminetetramethylenephosphonic acid (EDTPA), further referred to as r_PEZ, were studied as a support material for use in chromatography. Our previous studies have demonstrated the utility of r_PEZ in the separation of immunoglobulins from biological fluids. In the present study we sought to understand the(More)
Implanted medical devices are of increasing importance in the practice of medicine and it is estimated that more than 3-million people in the United States have long-term implants like vascular grafts, pacemakers, catheters and joint replacements. Even though most biomedical polymers employed are relatively inert, unreactive, and non-toxic, most implant(More)