A Sellström

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In rats treated with sodium cyanide (5-20 mg/kg, ip) dopamine was dose dependently decreased in the striatum within 60 sec. One of the main metabolites of dopamine in the central nervous system, 3-methoxy-4-hydroxyphenylacetic acid (HVA), was decreased in striatum, olfactory tubercle, and hippocampus. However, the oxidatively deaminated metabolite,(More)
The effects of sodium cyanide (NaCN) on the gamma-aminobutyric acid metabolizing enzymes glutamic acid decarboxylase (GAD) and gamma-aminobutyric acid transaminase (GABA-T) were studied in vitro. With no pyridoxal-5-phosphate added, GAD was non-competitively inhibited by NaCN, with an IC50 of 280 microM. GAD was also inhibited when exposed to an equimolar(More)
Primary astroglial cultures were grown from newborn rat cerebral hemispheres. Cultures, aged 7 and 14 days, were analysed with respect to their capacity to accumulate radioactive GABA and dopamine. Concentrative high-affinity uptake, showing Na+-dependence was observed for GABA where Km and Vmax were not significantly altered with age of the culture. No(More)
Primary cultures from rat or mouse brain hemispheres contain predominantly glial cells. These cells accumulated [3H]GABA and showed a specific fluorescence with FITC-labeled anti-S-100 antiserum. Round or elongated cells which reacted positively with antimacrophage antiserum and exhibited phagocytotic activity, were considered mesodermal macrophages. Big(More)
Brain primary cultures from newborn rat hemispheres have been further characterized. THe cultures contained the glial fibrillary acidic protein (GFAp; alpha-albumin), as analyzed quantitatively. GFAp (alpha-albumin) increased in concentration during growth of cultures and during maturation of the intact rat brain. FITC-labelled antibodies against GFAp(More)
Neuronal cultures were made from the 8-d-old embryonic chick telencephalon. The primary culture model was further improved, the medium composition was modified, and the cells grown for 10 d, which allowed the development of relatively differentiated neurones. A superfusion protocol was developed and applied to study the release of [3H]-gamma-aminobutyric(More)
In vivo microdialysis and EEG recording have been used in order to study the combined neurochemical and electrophysiological events during intoxication with soman (o-1,2,2-trimethylpropyl methylphosphono-fluoridate), a potent inhibitor of acetylcholinesterase (AChE), in the freely moving rat. All rats exposed to soman exhibited signs of AChE inhibition. The(More)