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U1-70K, a U1 snRNP-specific protein, and serine/arginine-rich (SR) proteins are components of the spliceosome and play critical roles in both constitutive and alternative pre-mRNA splicing. However, the mobility properties of U1-70K, its in vivo interaction with SR proteins, and the mobility of the U1-70K-SR protein complex have not been studied in any(More)
Kinesins and myosins are molecular motors that move on microtubules and actin filaments, respectively. These motor proteins are involved in a variety of cellular processes such as intracellular transport, spindle formation and chromosome segregation in eukaryotes (Langford, 1995; Hirokawa, 1998). Although kinesins and myosins hydrolyze ATP to generate force(More)
Calmodulin (CaM) is a calcium-binding protein that is involved in a variety of cellular processes, interacting with many proteins. Since many CaM interactions are calcium-dependent, they are difficult to detect using high-throughput methods like yeast-two-hybrid. Furthermore, detection of CaM binding sites requires a significant experimental effort. Using a(More)
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