Learn More
Porcine epidemic diarrhea virus (PEDV), which emerged in the United States in 2013, has spread throughout North America. Limited availability of PEDV complete genomes worldwide has impeded our understanding of PEDV introduction into the United States. To determine the relationship between the North American strains and global emerging and historic PEDV(More)
Fifteen 8-week-old conventional pigs were selected from a farm where pigs were suffering from postweaning multisystemic wasting syndrome (PMWS). Ten of the animals were diseased pigs showing typical signs of PMWS (wasting and respiratory disorders) and positive for infection with porcine circovirus type 2 (PCV2), and the other five animals selected as(More)
Although the quantities of organic compounds exuding from roots is not large, seldom exceeding 0.4% of the carbon photosynthesized, they do exert a very strong influence on the soil microorganisms and may be significant in affecting plant nutrient availability. There is evidence that exudates from the roots of some plants are toxic to roots of neighboring(More)
In February 2014, porcine deltacoronavirus (PDCoV) was identified in the United States. We developed a PDCoV real-time reverse transcription PCR that identified PDCoV in 30% of samples tested. Four additional PDCoV genomes from the United States were sequenced; these had ≈99%-100% nt similarity to the other US PDCoV strains.
The root exudates from seedlings of ten plant species grown under conditions of controlled environment and nutrition were biassayed for six vitamins of the B-group. Biotin was consistently present in the exudates in amounts sufficient to influence the growth of rhizosphere micro-organisms. Pantothenate and niacin were generally present, but usually at low(More)
Porcine epidemic diarrhea virus (PEDV) spread rapidly after being diagnosed in the USA in April 2013. In this study we assessed whether PEDV could become airborne and if so, whether the virus was infectious. Air samples were collected both from a room containing experimentally infected pigs and at various distances from the outside of swine farms(More)
The exudation of amino acids by the roots of tomato, subterranean clover, and phalaris growing in nutrient solution under different environmental conditions has been studied. The amounts and balance of amino acids exuded by the different plants differed — phalaris and tomato exceeding clover. Exudation was greater from the plants during the first two weeks(More)
Because porcine reproductive and respiratory syndrome virus (PRRSV) can be transmitted through semen, PRRSV-free boar studs need to be routinely monitored to rapidly detect any potential PRRSV introduction. However, current protocols for monitoring PRRSV in boar studs are diverse, sometimes very costly, and their effectiveness has not been quantified. The(More)
Porcine reproductive and respiratory syndrome virus (PRRSV) is an important pathogen of swine. The objective of the current study is to investigate the feasibility of using reverse transcription loop-mediated isothermal amplification (RT-LAMP) for the detection of PRRSV. The RT-LAMP is a recently described DNA amplification technique reported to be simple,(More)