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A spontaneous mutation of H-2Kb, Kbm29, was discovered among the progeny of F1 hybrid parents. Unlike other characterized spontaneous class I variants, this mutant was detected with the use of antibody, rather than tissue grafting. Although Kbm29 is serologically indistinguishable from the previously described mutant molecule Kbm3, it is identical to the(More)
The use of feeder based protocols for the creation, expansion and banking of hES cell lines are well established. The advances in technology for feeder free culture have predominantly relied on the cultivation of pluripotent cells in colony based systems, commonly used in conjunction with ill-defined matrices. We have developed a process to allow enzymatic(More)
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