A Otto-Bruc

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We seek to define the influence of retinal cGMP phosphodiesterase (PDE) on the GTPase activity of transducin (T). A novel stopped-flow/fast filtration apparatus [Antonny, B., et al. (1993) Biochemistry 32, 8646-8653] is used to deliver T alpha GTP free of rod outer segment (ROS) membranes to a suspension of phospholipid vesicles bearing holoPDE. As measured(More)
The gamma-subunit of the cGMP-phosphodiesterase (PDE gamma) of retinal rods forms a tight complex with the activated alpha-subunit of transducin (Gt alpha GTP gamma S). We observe that while PDE gamma is not the physiological effector of other G alpha subtypes, it can still detectably interact with them. This interaction is strong with Gi1 alpha and Gi3(More)
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