A. Ortega Ballesteros

Learn More
Laccases are multi-copper containing oxidases (EC 1.10.3.2), widely distributed in fungi, higher plants and bacteria. Laccase catalyses the oxidation of phenols, polyphenols and anilines by one-electron abstraction, with the concomitant reduction of oxygen to water in a four-electron transfer process. In the presence of small redox mediators, laccase offers(More)
BACKGROUND In the picture of a laboratory evolution experiment, to improve the thermostability whilst maintaining the activity requires of suitable procedures to generate diversity in combination with robust high-throughput protocols. The current work describes how to achieve this goal by engineering ligninolytic oxidoreductases (a high-redox potential(More)
The generation of diversity for directed protein evolution experiments shows an important bottleneck in the in vitro random mutagenesis protocols. Most of them are biased towards specific changes that eventually confer a predicted and conservative mutational spectrum, limiting the exploration of the vast protein space. The current work describes a simple(More)
The in vitro fermentation of several purified galacto-oligosaccharides (GOS), specifically the trisaccharides 4′-galactosyl-lactose and 6′-galactosyl-lactose and a mixture of the disaccharides 6-galactobiose and allolactose, was carried out. The bifidogenic effect of GOS at 1 % (w/v) was studied in a pH-controlled batch culture fermentation system(More)
Antioxidants protect cells against the effects of harmful free radicals and play an important role in preventing many human diseases (e.g. cancer, atherosclerosis, neurodegeneration, inflammatory disorders, etc.) and aging itself. In addition, antioxidant molecules are employed to prevent unsaturated oil products from becoming rancid during storage and thus(More)
A simple and inexpensive methodology, based on the use of micro-centrifuge filter tubes, is proposed for establishing the best enzyme immobilization conditions. The immobilized biocatalyst is located inside the filter holder during the whole protocol, thus facilitating the incubations, filtrations and washings. This procedure minimizes the amount of enzyme(More)
This work discusses the use of LAear Infrared Reflectance Spectroscopy (LAIRS) analysis with the object of studying the degree of degradation of stone material and other elements in cultural heritage monuments, as an alternative to traditional laboratory methods, with the advantage of being a fast non-destructive process that requires little or no sample(More)
In this paper we propose an approach to the production and maintenance of repositories of learning objects that is based in our previous experiences with the virtualization of two academic museums at Complutense University of Madrid. For this purpose we have adapted the specific learning object model and the incremental and evolutionary methodology used in(More)
Industrial development may result in the increase of environmental risks. The enzymatic transformation of polluting compounds to less toxic or even innocuous products is an alternative to their complete removal. In this regard, a number of different redox enzymes are able to transform a wide variety of toxic pollutants, such as polynuclear aromatic(More)
  • 1