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BACKGROUND AND PURPOSE The polyphenolic compounds present in green tea show cancer chemopreventive effects in many animal tumor models. Epidemiologic studies have also suggested that green tea consumption might be effective in the prevention of certain human cancers. We investigated the effect of green tea polyphenols and the major constituent,(More)
Cell surface 'blebbing' is an early consequence of hypoxic and toxic injury to cells. A rise in cytosolic free Ca2+ has been suggested as the stimulus for bleb formation and the final common pathway to irreversible cell injury. Here, using digitized low-light video microscopy, we examine blebbing, cytosolic free Ca2+, mitochondrial membrane potential and(More)
N-Methyl-D-aspartate causes a rapid increase in intracellular Ca2+ leading to collapse of the mitochondrial membrane potential and eventually cell death in cortical neurons. The aim of this study was to investigate the mechanism responsible for mitochondrial depolarization using laser scanning confocal microscopy of single cultured rate cortical neurons. To(More)
The complete AIF cDNA comprising the amino-terminal mitochondrial localization sequence (MLS) and the oxidoreductase domain has been fused in its carboxyl terminus to enhanced green fluorescent protein (GFP), thereby engineering an AIF-GFP fusion protein that is selectively targeted to the mitochondrial intermembrane space. Upon induction of apoptosis, the(More)
Exposure of rat hippocampal neurons or human D283 medulloblastoma cells to the apoptosis-inducing kinase inhibitor staurosporine induced rapid cytochrome c release from mitochondria and activation of the executioner caspase-3. Measurements of cellular tetramethylrhodamine ethyl ester fluorescence and subsequent simulation of fluorescence changes based on(More)
There is no clear picture of the critical events that lead to the transition from reversible to irreversible injury. Many studies have suggested that a rise in cytosolic free Ca2+ initiates plasma membrane bleb formation and a sequence of events that lead ultimately to cell death. In recent studies, we have measured changes in cytosolic free Ca2+,(More)
We studied if biomass, cell dimensions and microcystin concentration of the cyanobacterium Anabaena sp. (strain BIR 257; recently also referred to as Dolichospermum) change in the presence of turbidity caused by clay and a eukaryotic green algal species. Anabaena sp. was incubated in experimental lab bioassays, with added clay and the chlorophyte(More)
Collinear laser spectroscopy experiments with the Sc + transition 3d4s 3 D 2 → 3d4p 3 F 3 at λ = 363.1 nm were performed on the 42−46 Sc isotopic chain using an ion guide isotope separator with a cooler–buncher. Nuclear magnetic dipole and electric quadrupole moments as well as isotope shifts were determined from the hyperfine structure for five ground(More)
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