A N Kachman

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The response to electrophoretic application of acetylcholine and suberyldicholine was studied in two identified neurons (LPed-2 and LPed-3) isolated from Planorbarius corneus left pedal ganglion. Acetylcholine reversal potential was found to be significantly more negative with K(2)SO(4)-filled microelectrodes than with KCl-filled ones. Reversal potentials(More)
The patch-clamp technique in cell-attached configuration was applied to investigate suberyldicholine-induced currents in Planorbarius corneus neurons. The single channels currents registered as inward ones at hyperpolarization of the patches were due to chloride ions efflux from the cell. Suberyldicholine (5 mumol/l) was added into the patch electrode.(More)
In experiments on isolated neurones from the gastropod mollusc P. corneus, strophantin and digoxin in low concentrations produce slow hyperpolarization, in higher ones--depolarization; at concentrations about 1 mM, hyperpolarization was more evident. In all cases, the decrease in membrane resistance was observed. Presumably, membrane permeability for(More)
Changes in the membrane potential caused by acetylcholine or gamma-aminobutyric acid when added to the perfusion fluid were compared with those induced by their local application to the neuron soma membrane from the micropipette (diameter 100-300 mu). With equal drug concentrations, the responses were found to be of similar amplitude with either method of(More)
Patch-clamp technique in cell-attached configuration was applied to investigate ion permeability induced by suberyldicholine in the neurons of freshwater mollusc Planorbarius corneus. The inward currents through single channels were registered at patch potentials 50-100 mV more negative than resting potential of the cell. When the patch pipette contained(More)
Furosemide (2.10(-4) to 1.10(-3) g/ml) was shown to prevent the increase of chloride conductance induced in isolated neurons of freshwater mollusc Planorbarius corneus by ionophoretic application of acetylcholine, suberyldicholine or gamma-aminobutyric acid. Furosemide caused no shift in the reversal potential of the chloride-dependent responses if the(More)
Accumulation of acid fluorochrome in proximal tubuli in the frog surviving kidney has been investigated. Mean concentration of fluorochrome in the tubuli is linearly related to the duration of incubation. Changes in the mean rate of transport of fluorochrome into the tubuli induced by changes in its external concentration are satisfactorily described by(More)
In experiments on isolated and identified neurones of the pedal ganglion of the gastropod mollusc P. corneus, it was demonstrated that biphasic response to ACh may be obtained both to superfusion and electrophoretic application. Fast (depolarizing) phase may be imitated by a nicotinomimetic drug, suberyldicholine, and blocked by d-tubocurarine. These data(More)