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An antigen-capture ELISA was developed for the detection of pestivirus-specific antigens in peripheral blood leucocytes (PBLs), blood clots and tissue samples of immunotolerant cattle persistently infected with virus. The ELISA demonstrated complete agreement with conventional virus isolation procedures undertaken on specimens from a total of 58 carrier(More)
When 73 heifers (60 of which were seronegative to pestivirus) were inseminated with pestivirus-contaminated semen from a transiently infected bull, the conception rate to a single insemination was found to be normal (65 per cent). Only three animals became systemically infected, as determined by viraemia and seroconversion. Pestivirus was isolated from the(More)
During the certification of the bulls at an artificial breeding centre for freedom from pestivirus infection, a single viraemic bull was identified, and further testing confirmed that it was persistently infected. The two-year-old bull was healthy and of similar bodyweight to its peers. Its semen was of normal quality on the basis of density, motility and(More)
After the introduction of pestivirus into a herd undergoing an embryo transfer and artificial insemination program, substantial post-weaning calf losses occurred. The predominant clinical feature was severe respiratory disease, in contrast to the commonly recognised mucosal disease. Thirty-one of 76 calves were affected, with a case fatality rate of 58%.(More)
Since the war, developments in portable recording equipment have greatly assisted anthropologists, musicologists and other deeply concerned with the need to collect and preserve recordings of indigenous singing before it has been forgotten. In Australia and adjacent islands, commencing with the Australian-American expedition to Arnhem Land in 1948,(More)
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