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Most cellular processes are carried out by multiprotein complexes. The identification and analysis of their components provides insight into how the ensemble of expressed proteins (proteome) is organized into functional units. We used tandem-affinity purification (TAP) and mass spectrometry in a large-scale approach to characterize multiprotein complexes in(More)
Reconstituted transcription reactions containing the seven general transcription factors, in addition to RNA polymerase II, respond poorly to transcriptional activators. Two factors, Dr2 and ACF, necessary for high levels of transcription in response to an activator have been identified. ACF can enhance basal and activated transcription. Dr2 represses basal(More)
Recently, in situ protein microarrays have been developed for large scale analysis and high throughput studies of proteins. In situ protein microarrays produce proteins directly on the solid surface from pre-arrayed DNA or RNA. The advances in in situ protein microarrays are exemplified by the ease of cDNA cloning and cell free protein expression. These(More)
Reaching as high structural resolution as possible in 3D electron microscopy of biological specimens is crucial to understanding their function and interactions. Technical and biological limitations make electron microscopy projections of such specimens quite noisy. Under those circumstances, the broadly used Weighted Back-Projection algorithm presents some(More)
Reliable and timely fault detection and isolation are necessary tasks to guarantee continuous performance in complex industrial systems, avoiding failure propagation in the system and helping to minimize downtime. Model-based diagnosis fulfils those requirements, and has the additional advantage of using reusable models. However, reusing existing complex(More)
This paper shows the application of a 2DOF robust discrete dead-time compensator to a evaporation process in a sugar factory. The proposed controller is tuned using performance and robustness specifications and it is specially designed for integrative plants as is the case of the level control of the principal unit of the evaporator. A comparative analysis(More)
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