A M Houvenaghel

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The adhesion of K88ab, K88ac, K88ad, P987, K99, F41 and K99/F41 positive Escherichia coli strains to duodenal, jejunal and ileal villi was studied using an in vitro adhesion assay. The villi were harvested from 4- to 5-week-old pigs. The K88+ strains adhered in large numbers (42 +/- 5 to 81 +/- 4 E. coli/250 microns villous length) to the villi from most(More)
To induce diarrhoea and hypovolaemia, newly-weaned conventionally bred piglets (3- to 4-weeks-old), were either given secretagogues or were inoculated with enterotoxigenic Escherichia coli (ETEC). Choleratoxin (n = 2), E. coli heat-labile enterotoxin (n = 2) or castor oil (n = 2) were given intragastrically or piglets were intraperitoneally injected with DL(More)
In anesthetized piglets the influence of an LD100 of Escherichia coli endotoxin (0.5 mg/kg IV, bolus injection) on several hemodynamic parameters and on survival time was studied. Endotoxin provoked a pronounced decrease in arterial pressure and cardiac output and an increase in portal venous and pulmonary arterial pressure and heart rate. Total peripheral(More)
Aggregation of chicken thrombocytes was studied in whole blood using an electronic aggregometer. Serotonin (5-hydroxytryptamine, 5HT), arachidonic acid (AA) and collagen, but not adenosinediphosphate (ADP) induced aggregation. Prostaglandin (PG) endoperoxides were essential for arachidonic acid-induced aggregation, but were not involved in 5HT-induced(More)
In anaesthetized young pigs the influence of intra-arterial infusions of Substance P (SP) on mesenteric vascular resistance is studied, both before and after serotoninergic and nervous conductivity blockade by dihydroergotamine (DHE) and tetrodotoxin (TTX). SP has a strong dilator effect on pig mesenterial vessels, which is not significantly modified by DHE(More)
In laying hens the influence of prostaglandins (PG's), leukotrienes (LT's), VIP, carbachol and isoproterenol (IPN) on uterine and vaginal motility has been studied, using the techniques of the isolated organ-bath, of the intraluminal pressure registration in the anesthetized hen and of the electromyography in the conscious, non-sedated animal. The PG's,(More)
The phenotype of 21 weaned piglets, concerning adhesion of Escherichia coli possessing K88ab, K88ac or K88ad fimbriae to pig cells, was determined in an in vitro assay. Comparison was made with adhesion of these three K88 variant strains to buccal mucosal epithelial cells and to erythrocytes (haemagglutination) in the same piglets. Whereas adhesion of the(More)
The effect of an intravenous bolus injection of endotoxin, 0.1, 1 or 10 μg/kg, on rectal temperature, clinical appearance, haematological parameters, and on gastrointestinal electrical activity was examined in 11 conscious piglets of 4–5 weeks of age, with implanted electrodes in the antrum pylori, duodenum, jejunum and ileum. All doses resulted in a(More)
The influence of intravenous infusion of VIP, 150 and 300 pmol/kg/min, on gastrointestinal electrical activity was studied in conscious piglets with electrodes implanted in the wall of the antrum pylori, duodenum, jejunum, and ileum. Both doses resulted in a decrease in antral electrical activity. In the small intestine, only the lower dose caused a(More)
In newly-weaned 3–4 week old piglets (n=29) diarrhoea (100%) and vomiting (65%) were induced by inoculation with transmissible gastroenteritis virus and enterotoxigenic E. coli strains (0149:K91:K88ac; LT, STa and STb enterotoxin positive). This combined infection resulted in pronounced mortality within 7 days. During this period the piglets had decreases(More)