A K Jazova

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A metabolic selective system has been proposed for the selection of hybrid hybridomas (tetradomas) based on the introduction in one of the parental cell lines of two traits simultaneously--a recessive one (resistance to 8-azaguanine) and a dominant one (multidrug resistance). Tetradomas were selected in the presence of two selective agents: aminopterin and(More)
Monoclonal antibodies specific for human alpha-fetoprotein (AFP) were produced by the hybridoma technique. By using solid-phase immunofluorescence and radioimmunoassays, the antibodies were proven to distinguish 4 different epitopes on the AFP molecule.
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