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1. The fluorescent dye 5-N-hexadecanoyl-aminofluorescein (HAF) was used to study the mechanisms involved in maintaining a relatively constant luminal surface pH (pHs) in the distal colon of the guinea-pig. The fatty acyl chain of the HAF molecule inserts into the apical membrane of epithelial cells. This allows a continuous measurement of the surface pH for(More)
The response of the intracellular pH (pHi, measured with BCECF) of the caecal and distal colonic epithelium of guinea pig and of monolayers of HT29 clone 19a cells on the addition of short-chain fatty acids (SCFA) was assessed. Addition of SCFA to the luminal side of these cells had no major effect on pHi, independent of whether the apical Na+/H+ exchange(More)
A method for the continuous measurement of proton activity at the luminal surface of the gastro intestinal mucosa was developed. The pH-sensitive fluorescent dye 5-N-hexadecanoyl-amino fluorescein (HAF) was used to monitor the surface pH (pHs). HAF integrates in the apical membrane of the colonic cells because of its amphophilic character. By excitation at(More)
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