A Iu Kolosova

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The method of enzyme linked immunosorbent assay (ELISA) for quantitative detection of chloramphenicol (CAP) in human blood serum was developed. Peculiarities of the adsorption on the microtitre plates surface of CAP-ovalbumin conjugate were investigated. Different conditions of competition stage of the analysis were studied. Conditions providing CAP(More)
A quick reliable homogeneous polarization fluoroimmunoassay (PFIA) of progesterone was developed. The assay is carried out with Abbott TDx (USA) polarization fluorometer and it takes 5-7 min to analyze 10 samples by this method. The range of progesterone concentrations determined is 1 to 1000 ng/ml. Fluorescein labeled progesterone-3-carboxymethyloxime(More)
The method of solid-phase enzyme linked immunosorbent assay (ELISA) for quantitative detection of chloramphenicol (CAP) in milk was developed. Peculiarities of the adsorption on the microtitre plates surface of CAP-ovalbumin conjugate were investigated. Different conditions of competition stage of the analysis were studied. Conditions providing CAP(More)
An enzyme immune test system was designed and optimized for quantitative assay of gentamicin in human sera. Immunospecific reagents i.e. gentamicin conjugates with ovalbumin (for sorption on polysterol plates) and with bovine serum albumin (immunogen) were prepared. Gentamicin specific antisera were isolated and tested. Conditions for the antigen sorption(More)
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