A. I. Potapenko

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General properties of aging in animals are considered on the basis of the literature evidence and the results obtained by the authors of this paper. The existence of a specific aging mechanism is inferred. The operation of this mechanism is controlled not only by genes but also by particular noncoding genomic sequences with variable structure. The beginning(More)
Resonance light scattering, a phenomenon of an abrupt enhancement of Rayleigh light scattering in close proximity to an absorption band, is easily detectable in solutions of strongly absorbing chromophores, which form large aggregates with strong pi-pi-electronic coupling among the chromophores. Resonance light scattering spectra need to be corrected for(More)
Irradiation of the rabbit and human skin by near UV light (wave lengths greater than 320 nm) in the presence of 8-methoxypsoralen produced erythema, pigmentation and some changes in the mechanical and electric properties. All the photobiological effects mentioned were inhibited by alpha-tocopherol if it was applied before irradiation. When applied after(More)
Effect of antioxidants--alpha-tocopherol and 2,6-di-tert-butyl-hydroxytoluene (BHT) on 2-methoxypsoralen (8-MOP) photoaddition was investigated. Spectrophotometric experiments have shown that under anaerobic conditions alpha-tocopherol inhibits photodimerization of 8-MOP and photoaddition of 8-MOP to thymine (in 80% ethanol solution). 50% inhibition is(More)
The influence of gamma-irradiation and 5-bromo-2'-deoxyuridine (BUDR) on the life span and behaviour (in photoactivity test) of D. melanogaster was studied. It was found that gamma-irradiation resulted in decrease of life span. However the form of survival curves changed only under exposure to doses higher than 500 Gy. Treatment with BUDR distorted the form(More)
The amount of defects in DNA secondary structure recognized by nuclease S1 and their localization by sequences of various nucleotide composition were studied in relation to the age factor. It was established that a significant twofold increase in defects of DNA secondary structure was observed in hepatic cells of very old (30 months and older) intact mice(More)
In order to develop an adequate method for quantitative determination of age-related defects of DNA secondary structure in somatic cells, the testing of enzyme nuclease S1 ("Sigma") was carried out. The conditions have been selected under which this enzyme revealed all single-stranded breaks and did not induce nonspecific breaks in DNA. It was shown that(More)
Irradiation with near ultra-violet light is shown to lead to a decrease in the electric stability of liposome membranes. The presence of 8-methoxypsoralen during irradiation enhanced the effect. Vacuumization of the sample or presence of alpha-tocopherol inhibited the decrease of the electric stability. 8-methoxypsoralen is supposed to decrease the electric(More)