A I Korotiaev

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KMR plasmids controlling antibiotic resistance and the capacity for production of the colonization antigen were identified in wild strains of E. coli (026, 0126, 0124) and S. sonnei isolated from patients with acute intestinal diseases. The strains of E. coli 026 and E. coli 0126 carried p KMR207-1 plasmid determining resistance to chloramphenicol and(More)
Two restriction-modification systems specified by two plasmids are discovered in the clinical species of Shigella. The plasmids are designated pKMR114 and pKMR115. Both are of 60.800 bp and belong to the IncN incompatibility group. The EcoRI, EcoRV, HindIII restriction patterns of both plasmid DNAs are identical. As shown by efficiency of plating of(More)
The nucleotide composition of chromosome and plasmid DNA free of hybrid RNA isolated from resting Escherichia coli cells preliminary cultivated with the help of [14C] uracil has been studied. It has been established that DNA contains [14C]uracil side by side with adenine, thymine, guanine and cytosine. It confirms the presence of RNA fragments in the(More)
Shigella strains showed that in 43.9 per cent of the strains this feature was controlled by conjugative R plasmids. On the whole these were the plasmids allotting the bacterial cell with resistance simultaneously to SmTcCm (37.1 per cent) and SmTc (17.1 per cent). The plasmids with other phenotypes were less frequent: SmApCm, 11.4 per cent, TcApCm, 8.6 per(More)
DNA content was studied in Escherichia coli K12 cells carrying conjugative R plasmids. Cultures at the exponential growth phase were used for the fractionation of nucleic acids. The content of total and chromosomal DNA was found to be much lower in the presence of R plasmids than in the strain carrying no plasmids. The content of RNA also decreased in the(More)
DNA content was studied in Escherichia coli strains resistant to rifampicin and nalidixic acid. When the strain resistant to the acid was grown in the M-9 medium with glucose, it was found to contain 14.6 X 10(-15) to 17.8 X 10(-15) g of DNA per cell, the rifampicin-resistant strain contained 10.2 X 10(-15) to 34.8 X 10(-15) g of DNA per cell, and the(More)