A H Fenselau

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Vasoproliferative activity has been demonstrated in extracts of retinas from human, bovine, and feline sources. These retinal extracts are capable of stimulating (a) proliferation and thymidine uptake of bovine vascular endothelial cells in culture and (b) neovascularization on the chick chorioallantoic membrane. Extracts of skeletal muscle, cardiac muscle,(More)
A corneal micropocket assay for angiogenesis in the rat eye is described in detail. With our test system, a partially purified, low molecular-weight endothelial cell growth stimulatory factor isolated from the Walker 256 rat carcinosarcoma is demonstrated to have potent angiogenic activity in vivo. The advantages and applications of our rat corneal assay(More)
Intraocular neovascularization occurs in several ocular disorders. Evidence from clinical experimental observations suggests that the retina and the vitreous are potential sources of angiogenic stimulators and inhibitors which may play a role in the development of intraocular neonvascularization. We have demonstrated that extracts of adult retina are(More)
Succinyl coenzyme A:acetoacetate coenzyme transferase (EC 2.8.3.5), an initiator of ketone body usage and absent in normal liver, has been shown to be located in mitochondria from Morris hepatoma 7288ctc using differential and density gradient centrifugation. Furthermore, tumor mitochondrial subfractionation revealed that this transferase is associated with(More)
The amounts of succinyl-CoA--3-oxo acid CoA-transferase (EC 2.8.3.5) decrease progressively in skeletal muscle in streptozotocin-diabetic rats, reaching after 10 days about 50% of the value in normal rat muscle. Electrofocusing studies indicate the occurrence of partial proteolysis of the enzyme in diabetic muscle. However, several functional parameters(More)
We report here the purification of an angiogenic substance from the Walker 256 rat ascites tumor which is mitogenic for fetal bovine aortic endothelial cells in culture and which also stimulates new blood vessel growth in vivo. Purification was monitored in endothelial cell cultures by cell counting or by [3H]thymidine incorporation into DNA. Lyophilized(More)
Short-term cultures of endothelial cells from fetal bovine heart and aorta consistently display increased growth rates when crude tumor cell homogenates from the Walker 256 adenocarcinoma (ascites and solid forms as well as tumor cells from a suspension culture) are added to the culture media. The tumor-derived material produces growth stimulation in both(More)