A. García Castellano

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The skeletal muscle dihydropyridine receptor/Ca2+ channel is composed of five protein components (alpha 1, alpha 2 delta, beta, and gamma). Only two such components, alpha 1 and alpha 2, have been identified in heart. The present study reports the cloning and expression of a novel beta gene that is expressed in heart, lung, and brain. Coexpression of this(More)
Although pharmacological and electrophysiological studies have demonstrated the existence of multiple types of voltage-dependent calcium channels in neuronal tissue, the subunit composition of these channels is not well known. Here, we report the cloning and expression of a new rat brain beta subunit (beta 4). Northern blot analysis indicates that beta 4(More)
Phytotoxicity-based extraction and fractionation were employed to separate allelochemicals contained in an extract of Lethariella canariensis. Twelve phenolic substances were isolated from the phytotoxic fraction “Letharal” of the thalli. These were identified by spectroscopic methods, physicochemical constants, and HPLC chemical correlation, and determined(More)
1. We have studied the effect of external K+ and tetraethylammonium (TEA) on several mutants of Shaker B K+ channels with amino acid substitutions in the pore which alter TEA affinity and the rate of C-type inactivation. In all channels studied high external K+ makes C-type inactivation slower. 2. In the wild-type channel, TEA blockade is voltage dependent(More)
Voltage-gated Na+ and Ca2+ conductances of freshly dissociated septal neurons were studied in the whole-cell configuration of the patch-clamp technique. All cells exhibited a large Na+ current with characteristic fast activation and inactivation time courses. Half-time to peak current at -20 mV was 0.44 +/- 0.18 ms and maximal activation of Na+ conductance(More)
The Ca(2+)- and voltage-dependent K+ (maxi-K) channel beta(1)-subunit mRNA is particularly abundant in cardiomyocytes but its functional role is unknown. This is intriguing because functional maxi-K channels are not found in cardiomyocyte plasmalemma, although they have been suggested to be in the inner mitochondrial membrane and participate in(More)
The diversity of L-type calcium channels was probed using the polymerase chain reaction and primers based on regions conserved in the L-type skeletal muscle (CaCh 1) and cardiac calcium channels (CaCh 2). Related sequences were amplified from human heart, hamster heart, rabbit heart, mouse ovary, mouse BC3H1 cells, and hamster insulin-secreting (HIT) cells.(More)
The skeletal muscle dihydropyridine receptor/Ca2+ channel consists of five distinct subunits (alpha 1, alpha 2 delta, beta 1, and gamma). Homologous alpha 1, alpha 2 delta, and beta 2 subunits are expressed in heart and brain. The present study reports the cloning and expression of a third beta subunit, beta 3, which is expressed predominantly in brain. Its(More)
Miguel Fernández-Tenorio, Cristina Porras-González, Antonio Castellano, Alberto del Mechanism for Depolarization-Evoked Mammalian Arterial Contraction Channels : New 2+ Metabotropic Regulation of RhoA/Rho-Associated Kinase by L-type Ca Print ISSN: 0009-7330. Online ISSN: 1524-4571 Copyright © 2011 American Heart Association, Inc. All rights reserved. is(More)
Introduction Voltage-gated Ca2+ channels have been extensively characterized in terms of their pharmacological, electrophysiological, biochemical, and molecular biological properties. Calcium channels are multisubunit complexes composed of an ion-conducting subunit, a ,, and smaller accessory subunits. Molecular cloning studies have concentrated on the a ,(More)