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Journals and Conferences
In this review the main principles and possibilities of a hybridization analyses as a highly specific methods for diagnostics of infections and genetic diseases are discussed. Special attention is paid to the chemical approaches to the preparation of non-radioactive probes based on synthetic oligonucleotides and polynucleotides containing biotin, Eu3+ and… (More)
The directed chemical synthesis of three pentadeoxyribonucleotides, dpCpTpApGpA, dpTpCTpApG, and dpApApApCpC, corresponding to the 5′-terminal segment of yeast valine tRNA, has been effected.
The chemical approaches to the preparation of non-radioactive and biotinylated probes based on synthetic oligonucleotides and their applicability to the molecular hybridization method are discussed.
The decadeoxynucleotide d(pCCACGAAACC) transcription by E. coli RNA-polymerase was studied. The transcript was shown to be several times longer than the template. The oligonucleotide GpGpGpGpUp complementary to the "contact" of two neighbouring template molecules was found in the pancreatic RNase digest of the RNA-product. This fact is consistent with our… (More)