A. -F. Holstein

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Ganglia from Auerbach's plexus of the large intestine (caecum, appendix vermiformis, colon transversum and rectum) in man, rhesus monkey and guinea-pig are composed of nerve cells and their processes, typical Schwann cells and a vast neuropil. The neuropil consists of dendrites and axons of intrinsic nerve cell perikarya and axons of extrinsic neurons.(More)
In the human testis the formation of the residual body of the spermatid and its morphological changes during and after spermiation were studied by means of electron microscopy. The caudal cytoplasmic mass of the late spermatid contains a Golgi complex, mitochondria, annulate lamellae, a chromatoid body, flower-like structures, ribosomes, a few large(More)
1. The localization of catecholamines and 5-hydroxytryptamine has been investigated in the human testis, epididymis, ductus deferens and prostate obtained at operations on patients aged 26–79 years. 2. In fluorimetric determinations noradrenaline was found to be present in varying concentrations in the genital tract. The lowest amount (0.07 μg/g) was found(More)
Throughout spermatogenesis multiplication, maturation and differentiation of germ cells results in the formation of the male gamete. The understanding of spermatogenesis needs detailed informations about the organization of the germinal epithelium, the structure and function of different types of germ cells, endocrine and paracrine cells and mechanisms,(More)
Malformed spermatids and spermatozoa in the testes, epididymes, and ejaculates of 29 apparently healthy men and in 171 patients suffering from different andrological diseases were investigated with the electron microscope. The following types of malformations are described: 1) Malformations of the acrosome in contact with the nucleus of the spermatid:(More)
Nerve growth factor (NGF) affects morphology and function of isolated and cultured seminiferous tubules from human testis. Quanitative determination of this neurotrophic protein revealed an amount of 5.4 ng per g human testis, suggesting a crucial function of NGF in spermatogenic tissue. With the use of immunohistochemical methods target cells for NGF were(More)
The spermatogenesis in the spiny dogfish (Squalus acanthias) was investigated by means of light- and electronmicroscopy. In a single histologic section the reproduction and differentiation of germ cells — located in so called ampullae — can be studied, proceeding from early spermatogonial cells up to mature spermatozoa. 9 zones of ampullae in different(More)
A number of marker substances for neuronal and neuroendocrine cells have been demonstrated in the cytoplasm of the interstitial Leydig cells of human testes using basic immunocytochemical methods and some of their modifications. We were able to reveal immunoreactivity for enzymes involved in the synthesis of the catecholamines dopamine and noradrenaline(More)
Numerous mitoses were noted in testicular tissue from adult men with early germ cell tumors. More than 15 Leydig cells undergoing mitosis were found in the interstitial compartment. The presence of specific crystalline intracytoplasmatic inclusions demonstrated for the first time that differentiated Leydig cells are capable of proliferation. Occasionally(More)
Die Ductuli efferentes des normalen und kastrierten Kaninchenbockes wurden licht- und elektronenmikroskopisch untersucht. Beim gesunden, geschlechtsreifen Tier ist das Epithel zweireihig und enthält zwei Zelltypen. 1. Typ I: Zellen mit basalem Kern. Sie enthalten ein girlandenförmiges Ergastoplasma, zahlreiche Mitochondrien, Cytosomen und apikal gelegene(More)