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In order to study the surface behaviour of puroindoline-a (PIN-a) in the presence of lipid monolayers, protein solutions were injected beneath films of the polar wheat lipid diacylgalactosylglycerol… (More)
The lipid-binding properties of wheat puroindolines (PINs) make these proteins likely candidates to play an important role in the stability of lipid films in the gas cells of bread dough, an… (More)
In addition to the Ca2+ ion at the active site, porcine pancreatic phospholipase A2 (PLA) is known to bind a second calcium ion with a lower affinity at alkaline pH. The second calcium-binding site… (More)
The cDNA coding for porcine pancreatic prophospholipase A2 (proPLA) has been cloned and expressed in Saccharomyces cerevisiae. Expression and secretion of proPLA could only be obtained after fusing… (More)
The structure of the central repetitive domain of high molecular weight HMW) wheat gluten proteins was characterized in solution and in the dry state using HMW proteins Bx6 and Bx7 and a subcloned,… (More)