A. Bluma

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To observe and control cultivation processes, optical sensors are used increasingly. Important variables for controlling such processes are cell count, cell size distribution and the morphology of cells. Among turbidity measurement methods, imaging procedures are applied for determining these process values. A disadvantage of most previously developed(More)
To observe and control cultivation processes, optical sensors are used increasingly. Important parameters for controlling such processes are cell count, cell size distribution, and the morphology of cells. Among turbidity measurement methods, imaging procedures are applied for determining these process parameters. A disadvantage of most previously developed(More)
Animal cell culture is widely used in biotechnology for the production of many biological products. In situ microscopes acquire images directly from cell suspensions and analyze the images in matters of cell concentration, cell size distribution and cell morphology. Their applicability was already proven for yeast and suspended mammalian cell cultivations.(More)
Over the last two decades, more and more applications of sophisticated sensor technology have been described in the literature on upstreaming and downstreaming for biotechnological processes (Middendorf et al. J Biotechnol 31:395-403, 1993; Lausch et al. J Chromatogr A 654:190-195, 1993; Scheper et al. Ann NY Acad Sci 506:431-445, 1987), in order to improve(More)
An algorithm is presented that is able to detect the regions which correspond to the single isolated human insulin crystals in a group of previously segmented foreground regions. It is based on a single nearest prototype rule, which requires the knowledge of a class prototype for each class of segmented foreground regions. Each class prototype represents a(More)
In this contribution, an algorithm is presented, which is able to extract the shortest linear edge and the longest diagonal of each single isolated human insulin crystal region, which is found in an arbitrary image captured by an insitu microscope inside of a reactor, where a human insulin crystallization processes is taking place. First, the image regions(More)
This article deals with the use of pyranose oxidase (PyOx) and glucose oxidase (GOx) enzymes in amperometric biosensor design and their application in monitoring fermentation processes with the combination of flow injection analysis (FIA). The amperometric studies were carried out at -0.7 V by following the oxygen consumption due to the enzymatic reactions(More)
A highly stable and sensitive amperometric alcohol biosensor was developed by immobilizing alcohol oxidase (AOX) through Polyamidoamine (PAMAM) dendrimers on a cysteamine-modified gold electrode surface. Ethanol determination is based on the consumption of dissolved oxygen content due to the enzymatic reaction. The decrease in oxygen level was monitored at(More)
The automated monitoring of cell variables in cultivation processes is a key technology in modern bioscience. In this article an innovative analytical tool for monitoring of cell densities in shaking-flask cultivations--consisting of a flow-through microscope with an automated image analysis software integrated in a FIA sampling system--is presented. This(More)
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