A Bakardjiev

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Uptake of beta-alanine and synthesis of carnosine (beta-alanyl-histidine) could be demonstrated in primary cell cultures derived from embryonic chick pectoral muscle. Concomitant with the morphological changes, cessation of cell division and the induction of creatine kinase, a rapid increase in the rate of beta-alanine uptake and also in the rate of(More)
Although the recently identified ICOS/B7h costimulatory counterreceptors are critical regulators of CD4(+) T cell responses, their ability to regulate CD8(+) responses is unclear. Here we report using a tumor-rejection model that ectopic B7h expression can costimulate rejection by CD8(+) T cells in the absence of CD4(+) T cells. Although responses of naive(More)
The effect of prostaglandin E1 (PGE1) on temperature-induced membrane lipid phase transitions of human blood platelets was investigated using excimer forming 1-pyrenedecanoic acid as fluorescent probe. PGE1 and PGF2 alpha at 10 microM concentrations had a small but reproducible effect on the melting curves between 20 degrees and 30 degrees C and lowered the(More)
Cultures of glial cells consisting predominantly of oligodendrocytes and astrocytes were prepared to study whether the biosynthesis of carnosine (beta-Ala-His) and the cellular uptake of this dipeptide are processes which are associated with a specific cell type. Uptake of the radiolabeled precursor beta-alanine was observed in both cultures. Synthesis of(More)
Primary cultures of glia cells obtained from adult rat olfactory bulb synthesize carnosine (beta-alanyl histidine). The rate of synthesis increases the older the culture is and is enhanced by the addition of dibutyrylcyclic-AMP (dBcAMP) to the medium. Millimolar concentrations of this agent intensify galactocerebroside (GalC) staining compared to control(More)
Biosynthesis, release, and uptake of carnosine (beta-alanyl-L-histidine) in highly enriched primary cell cultures of skeletal muscle and CNS tissue have been investigated. The synthesis is restricted to muscle cells, oligodendrocytes, and ensheathing cells of olfactory bulb and increases during differentiation of these cells. Astrocytes, in contrast, do not(More)
Oligodendrocytes obtained from rat brain 0-2 A progenitor cells and differentiated in culture take up beta-alanine and synthesize carnosine (beta-Ala-His). The present study was designed to determine whether carnosine and beta-alanine are released from such cultures in response to some stimuli. An evoked release of these substances was not observed when the(More)
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