A. B. Lunkina

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Rat hepatoma cell line McA Rh 7777 produces α-fetoprotein (AFP)in vitro. This line is subcloned into ATP-producing and nonproducing subclones. The AFP-negative clone is used to generate hybridomas producing monoclonal antibodies recognizing AFT− and AFP+ clones. One of these hybridomas (A2/3) reacts only with AFP− clones. Double staining for AFP and A2/3(More)
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