A Arevalillo

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The low Zn2+ complex formation constants, the capacity to degrade penicillin G in combination with Zn2+, and UV absorbance make 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, 3-[N-tris(hydroxymethyl)methylamino]-2-hydroxypropanesulfonic acid, 1,3-bis[tris(hydroxymethyl)methylamino]propane, and 1,4-piperazinediethanesulfonic acid convenient buffers to(More)
The hydrolysis of P-lactam antibiotics to yield products that are innocuous to the bacteria is catalyzed by P-lactamases (EC 3.5.2.6) (1, 2, 8). The enzymatic action modifies the UV spectrum of the antibiotic and also liberates protons. Most of the continuous assays of P-lactamases currently used are based on monitoring one of those events, although the(More)
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