A A Selianko

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Tubocurarine (Tc) effect on membrane currents elicited by acetylcholine (ACh) was studied in isolated superior cervical ganglion neurons of rat using patch-clamp method in the whole-cell recording mode. The "use-dependent" block of ACh current by Tc was revealed in the experiments with ACh applications, indicating that Tc blocked the channels opened by ACh.(More)
Membrane conductance activated by acetylcholine (ACh-conductance) was studied in rat isolated superior cervical ganglion neurons by means of the patch-clamp method in the whole-cell recording mode. It was found that ACh-conductance was increased or decreased with membrane hyper- or depolarization, respectively. The decrease in ACh-conductance was not(More)
The actions of bis-ammonium compounds on acetylcholine-activated channels were studied in voltage-clamped neurons of isolated superior cervical ganglion in a rabbit. The kinetics of the compound binding to open channels was estimated from shortening of the decay of fast excitatory postsynaptic current (which is determined by the rate of channels closure).(More)
Effect of alpha-neurotoxins from snake venoms (alpha-bungarotoxin and alpha-cobratoxin, 10(-6)M) on the ACh-induced current and on the fast excitatory postsynaptic current (EPSC) were studied in voltage-clamped neurons of the isolated rabbit superior cervical ganglion treated with atropine (10(-6)M) alpha-Neurotoxins produced potentiating or inhibitory(More)
The effect of iontophoretically applied acetylcholine and serotonin (5-hydroxytryptamine) on the neurons of the rabbit isolated superior cervical ganglion was studied by means of intracellular recording. It is found that the ganglion cell soma has only nicotinic receptors and no muscarinic receptors; nicotinic receptors are dispersed nonuniformly on the(More)
Ionic mechanisms of EPSP and depolarization evoked by iontophoretically applied acetylcholine (Ach) and serotonin (5-hydroxytryptamine, 5-HT) in the neurons of the rabbit isolated superior cervical ganglion (ACh-potential and 5-HT-potential, respectively) were studied using intracellular electrodes. Reversal potentials of EPSP (EEPSP) and Ach-potential(More)
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