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为了解植物类固醇5ɑ-还原酶基因( DET2 )作为油菜素内酯(BR)生物合成途径中限速酶的作用。利用RACE技术克隆获得棉花的 GhDET2 基因,并进行生物信息学分析,然后对棉花幼苗使用PEG6000模拟干旱胁迫24h,进行不同浓度油菜素内酯(Brassinolide,BR)的喷施处理,分析 GhDET2 基因在棉花幼苗根、茎、叶中的表达情况。序列分析表明 GhDET2Continue Reading